The polypeptide of interest was purified by Ni2+ affinity chromatography under denaturing conditions, followed by renaturation of the recombinant protein by dialysis, and digestion with enterokinase to release the chimeric peptide.

1、

Objective To establish a prokaryotic expression system for expressing mouse enterokinase which could recognize and cut the sequence of ( Asp)_4-Lys in order to provide a technique platform for application of recombinant enterokinase.

目的：制备能在原核表达系统中表达并特异性识别（Asp）4-Lys序列的鼠源性肠激酶，为推广应用重组肠激酶提供技术平台。

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2、

Trypsin exists in fish's pancreas with inactive zymogen, which is activated by enterokinase or trypsin, and its molecular structure has changed from trypsinogen to trypsin.

胰蛋白酶是以无活性的酶原形式存在于鱼类胰脏中，被肠激酶或有活性的胰蛋白酶自身激活，分子构象发生一定改变后转变为有活性的胰蛋白酶。

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3、

We obtain a high titer recombinant phage by using Enterokinase cleavage. It indicate that the residues in N-terminal of p ⅷ may prevent mimotope binding with antibody.

利用肠激酶酶切该重组噬菌体，获得了高滴度的重组噬菌体，证明了模拟表位N末端的噬菌体蛋白残余序列会妨碍其与OTA抗体的结合。

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4、

By SDS-PAGE, we found that the protein band of oil body changed significantly after digestion by enterokinase, indicated that enterokinase was effective to cleave purified oil body.

SDS-PAGE凝胶电泳发现，转基因油菜油体肠激酶酶切前后蛋白条带发生了明显改变，说明肠激酶可以对纯化的油体进行有效切割。

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5、

Enterokinase ( EK) is one of the most useful tools as a fusion protein cleavage regent for purification of recombinant protein production in biopharmaceutical industry.

肠激酶（Enterokinase，EK）是目前生物制药领域纯化重组蛋白产品时用于切割融合蛋白的首选工具酶之一。

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6、

Apart from normal specific cleavage and nonspecific cleavage, a possible sub-specific site was discovered in cleaving a fusion protein with enterokinase;

在酶切融合蛋白时，除正常切割和非特异性切割以外，发现了牛肠激酶的一个可能的次特异性识别序列。

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7、

Enterokinase ( EK) is a serine proteinase which consists of a high chain and a light chain connected by a disulfide bond.

Enterokinase（EK）是哺乳动物消化系统中最基础的丝氨酸蛋白水解酶之一，由重链和轻链组成，两条链通过一对二硫键相连。

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8、

Purification and Application of Bovine Enterokinase Catalytic Subunit ( EK_L) Expressed from the Cloned cDNA Encoding EK_L

牛肠激酶催化亚基cDNA的克隆、表达及表达产物的纯化和应用

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9、

Recombinant hKS with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

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10、

Objective: To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins.

研究目的：克隆表达人肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化。

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11、

Coli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized.

结论：鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达，天然构象重组肠激酶的获得还需对表达系统进行优化。

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12、

Several small fragments formed after cleavage by enterokinase. More researches on qualitative analysis and purification are progressing.

酶切后生成多个小分子量片段。定性分析及分离纯化工作仍在继续。

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13、

The purified enterokinase reached a purity of above 80% by SDS-PAGE analysis and showed high activity at fusion protein.

经SDS-PAGE测定，纯化的重组肠激酶纯度达80%以上，并能高效切开融合蛋白。

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14、

Construction, High-Density Fermentation and Purification of Recombinant Enterokinase Catalytic Subunit in Pichia pastoris

牛肠激酶基因工程菌的构建、高密度发酵、纯化及活性鉴定

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15、

The optional high cell-density fermentation conditions, purification methods and biological activity of the recombinant enterokinase in E.

对重组肠激酶在大肠杆菌中的高密度发酵表达条件、纯化方法及其生物活性测定进行了研究。

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16、

Enterokinase Cleavage of Fusion Proteins for Preparation of Recombinant Human Parathyroid Hormone 1& 34

利用肠激酶加工融合蛋白的方法制备重组人甲状旁腺素（1～34）肽

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17、

Refolding of the Fusion Protein of Recombinant Enterokinase Light Chain rEK_L

融合牛肠激酶轻链EK L包涵体蛋白的复性纯化

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18、

Fermentation and Purification of Recombinant Enterokinase by Producing in E.coli

重组肠激酶在大肠杆菌中的发酵与纯化

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19、

The influence factors for the high-level expression of recombinant enterokinase in the Pichia pastoris

重组肠激酶在甲醇酵母中高效表达的培养条件研究

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20、

The Study of Enterokinase Produced by Recombinant E.coli

重组大肠杆菌生产肠激酶的研究

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