Recombinant hKS with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

1、

Objective: To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins.

研究目的：克隆表达人肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化。

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2、

Coli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized.

结论：鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达，天然构象重组肠激酶的获得还需对表达系统进行优化。

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3、

Several small fragments formed after cleavage by enterokinase. More researches on qualitative analysis and purification are progressing.

酶切后生成多个小分子量片段。定性分析及分离纯化工作仍在继续。

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4、

The purified enterokinase reached a purity of above 80% by SDS-PAGE analysis and showed high activity at fusion protein.

经SDS-PAGE测定，纯化的重组肠激酶纯度达80%以上，并能高效切开融合蛋白。

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5、

Construction, High-Density Fermentation and Purification of Recombinant Enterokinase Catalytic Subunit in Pichia pastoris

牛肠激酶基因工程菌的构建、高密度发酵、纯化及活性鉴定

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6、

The optional high cell-density fermentation conditions, purification methods and biological activity of the recombinant enterokinase in E.

对重组肠激酶在大肠杆菌中的高密度发酵表达条件、纯化方法及其生物活性测定进行了研究。

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7、

Enterokinase Cleavage of Fusion Proteins for Preparation of Recombinant Human Parathyroid Hormone 1& 34

利用肠激酶加工融合蛋白的方法制备重组人甲状旁腺素（1～34）肽

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8、

Refolding of the Fusion Protein of Recombinant Enterokinase Light Chain rEK_L

融合牛肠激酶轻链EK L包涵体蛋白的复性纯化

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9、

Fermentation and Purification of Recombinant Enterokinase by Producing in E.coli

重组肠激酶在大肠杆菌中的发酵与纯化

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10、

The influence factors for the high-level expression of recombinant enterokinase in the Pichia pastoris

重组肠激酶在甲醇酵母中高效表达的培养条件研究

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11、

The Study of Enterokinase Produced by Recombinant E.coli

重组大肠杆菌生产肠激酶的研究

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12、

Expression of recombinant mouse enterokinase light chain in E.coli

重组鼠源性肠激酶轻链在大肠杆菌中的表达

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13、

Secretory Expression, Purification and Characterization of Bovine Enterokinase Light Chain

牛肠激酶轻链在毕赤酵母中的分泌表达、纯化和活性鉴定

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14、

Characterization of Enterokinase and Its Research Advances in Genetic Engineering

肠激酶特点及其基因工程的研究进展

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15、

Consulting the epidemiological conditions, clinical signs, and pathological observations, this results confirmed that, this disease is an enterotoxemia of piglets caused by clostridium perfrigens type A and C.

结合着流行病学情况，临床症状和病理学观察结果，肯定了此种疾病即是由产气荚膜梭菌A型和C型引起的一种仔猪肠毒血症。

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16、

STUDIES ON ENTEROTOXEMIA IN PIGLETS& ⅱ. Vaccine and Immunization

仔猪红痢的研究&Ⅱ、菌苗研究及免疫试验

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17、

Conclusion The chief method to diagnose the children acute indigitation is abdomen B-ultrasound, the air clyster is the next in order.

结论小儿急性肠套叠首选诊断方法为腹部B超，其次为空气灌肠。

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18、

Treatment and Nursing of 5 388 Children with Indigitation dy Hydraulic Clyster urder B-ultrasonographic Monitoring

B超监视下水压灌肠小儿肠套叠5388例的复位治疗与护理

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19、

Objective To evaluate diaplastic indication of children indigitation with air clysis and to increase diaplastic efficiency.

目的探讨小儿肠套叠空气灌肠复位的指征以及如何进一步提高整复率。

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20、

Objective To explore the representation and significance on abdomen plain film after infant indigitation to avoid misdiagnosis.

目的探讨婴儿发生肠套叠后，腹部X线平片的表现及意义。

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