renaturation

Renaturation of high concentration lysozyme using gel filtration chromatography assisted by artificial chaperones

人工伴侣与凝胶过滤色谱耦合作用促进高浓度溶菌酶复性研究

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Effect of Induction Time on Quality and Renaturation of Inclusion Bodies with Procarboxypeptidase B

诱导时机对羧肽酶原B包涵体质量和复性率影响的研究

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After purification with the column of Ni2+-NTA and renaturation.

利用Ni2+-NTA柱纯化和复性。

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The polypeptide of interest was purified by Ni2+ affinity chromatography under denaturing conditions, followed by renaturation of the recombinant protein by dialysis, and digestion with enterokinase to release the chimeric peptide.

经表达条件优化，大量制备重组蛋白，变性条件下进行镍离子亲和层析纯化。对纯化后的重组蛋白进行复性，用肠激酶切割复性蛋白以获得目的多肽。

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The conditions of purification, denaturation and renaturation of recombinant Bovine growth hormone ( BGH) were researched in order to get a good renaturation result.

为了取得较好的复性效果，对重组牛生长激素的纯化及变复性条件进行了研究。

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Cloning and Expression of the Thermolabile Hemolysin Gene of Vibrio Parahaemolyticus, Study on Renaturation and Functions of Its Expression Protein

副溶血弧菌不耐热溶血毒素基因的克隆、表达及表达蛋白的复性和功能的研究

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After renaturation, AIF protein binded DNA and induced nuclear apoptosis.

经EMSA与 Hoechst33258 染色实验证实:获得的AIF蛋白具有良好地与DNA结合并可诱导肿瘤细胞核凋亡的能力,凋亡率为37%.

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The experimental conditions, including temperature, pH, denatured protein concentration and urea concentration, in the renaturation process of recombinant human interferon-γ inclusion body by dilution were studied and at the same time pulse renaturation was tested. The optimum renaturation conditions were obtained.

采用稀释法研究了重组人γ干扰素包涵体复性条件，如温度、pH值、蛋白浓度、复性液中脲浓度，同时对加样操作方式进行了考察，得到了较为适宜的复性条件。

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And also the presence of DTT in renaturation solution maybe had no influence on the lysozyme refolding yields.

复性液中DTT的存在对复性效果没有影响。

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Renaturation and Mass Spectrometric Analysis of Chemically Synthesized ω-conotoxin M ⅶ A

化学合成ω-芋螺毒素MⅦA的复性与质谱分析

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The means of chromatographic renaturation can renature and purify the protein at the same time, thus simplify the procedure of operation and promote the recovery ratio of the product.

色谱复性使蛋白质的复性与纯化同步进行，简化了操作程序，提高了产品的回收率。

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After purification, the target protein further renaturation 2-BMP via degeneration dissolving, load dialysis bag.

纯化后的的目的蛋白进一步复性,BMP-2经变性液溶解, 装入透析袋内.

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A heterozygous sample after denaturation, renaturation of PCR, contains four duplexes: two homoduplexes and two heteroduplexes.

杂合子样品经过PCR变性、复性之后，其组分包含四种双链分子：两种同源双链、两种异源双链。

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