collagenase

Methods Chondrocytes were isolated from articular cartilage of 2-week-old rabbit, digested by collagenase ⅱ, and mixed with bovine collagen I, human blood freeze-dry fibrinogen and thrombosin according to a certain proportion, and were cultured in vitro.

方法：取2周龄的新生兔关节软骨，经消化，将获得的软骨细胞与牛型胶原、人血冻干纤维蛋白原、凝血酶按一定比例混合，制成软骨培养物并在体外培养。

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Methods: Using different techniques of collagenase digestion and discontinued density gradient centrifugation adultrat islets were prepared.

方法采用不同的胶原酶消化法及不连续密度梯度纯化法.

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methods sheep lumbar intervertebral disk displacement and degeneration 10 weeks before intradiscal injection of collagenase.

目的研究胶原酶盘内注射对山羊腰椎间盘突出模型的化学髓核溶解作用,探讨化学髓核溶解术后疼痛反应的原因.

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Results The cementum on root surface showed synchronously-happened destruction after combined attack by acid and collagenase.

结果经酸性胶液和胶原酶联合攻击的根面牙骨质呈现脱矿与基质胶原纤维破坏同步发生的超微结构表现。

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Conclusion Cutting and collagenase digestion process was easy, to obtain thymic epithelial cells of BXSB mice. We established a stable thymic epithelial cells culture system of systemic lupus erythematosus mice.

结论剪切、胶原酶消化法简便易行，可获得符合实验要求的BXSB小鼠胸腺上皮细胞，建立了稳定的系统性红斑狼疮模型鼠胸腺上皮细胞培养体系。

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helps restricts the formation of collagenase, prevent collagen and thus maintain skin's elasticity.

能抑制胶原蛋白酵素, 避免肌肤真皮层中胶原蛋白的断裂,保护肌肤弹性.

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we considered ct number of the disc as an objective index for prognosis of collagenase chemonucleolysis.

我们提出用CT值量化突出椎间盘的硬度,预测胶原酶溶解术后疗效.

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Multipotent adipose-derived MSCs were isolated from subcutaneous, ovarian, or circumrenal adipose tissue of rabbit according to our method. Briefly, minced adipose tissue were digested with 0.1% collagenase type I and 1% BSA for 90 min at 37 ℃ with intermittent shaking.

首次采用O.1%胶原酶型I+1%BSA，消化90min的分离方法，从家兔腹股沟皮下脂肪组织，卵巢脂肪组织，或肾脏周围脂肪组织中分离得到脂肪MSCs。

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effects of diammonium glycyrrhizinate on the lipid peroxidation and interstitial collagenase activity in fibrotic liver in rats

甘草酸二铵对肝纤维化大鼠肝脏脂质过氧化与间质性胶原酶活性的影响

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Islets were isolated by collagenase digestion and were transplanted under the left kidney capsule.

采用胶原酶消化法分离胰岛并移植到左肾被膜下.

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ich models were produced with the induction of collagenase type-s except normal group.

除正常组外,均用 -S 型胶原酶诱导大鼠脑出血模型.

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conclusion collagenase could prevent adhesion of intrathecal tendon without affecting tendon healing.

结论胶原酶有减轻肌腱鞘内粘连的作用,对肌腱的愈合无影响.

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OBJECTIVE To prepare the collagenase immunizing conjugation product for injection.

目的:制备注射用胶原蛋白酶免疫结合物.

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objective to investigate the causes of the initial nerve roots lesion after collagenase chemonucleolysis.

目的通过动物实验探讨胶原酶溶盘术后早期出现神经根病损原因.

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The influence on the numerical. count of Schwann cell ( SCs) from sciatic nerve and spinal ganglion was compared among various methods of enzymatic digestion technique, as homogenization or not and different enzymes ( trypsin, collagenase or a mixture of them) with various concentrations.

作者用酶消化的方法分离雪旺细胞（Schwann Cell，SCs），比较了匀浆与否，及不同浓度的胰酶、胶原酶、胰酶／胶原酶混合液对不同材料所获分离SCs数量的影响。

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objective to isolate and purify collagenase with high quantity and activity.

目的分离获得高产量、高比活性的胶原酶.

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Methods The average dissecting force ( ADF) and interlamellar shear stress of each interspace in the media were measured using a tensile strength tester, and the the final shape-changed strength ratio of each layer was calculated before and after melting the tissue with collagenase and elastase.

方法测定人主动脉中膜各层间单位分离力、剪应力和胶原酶和弹力蛋白酶酶解前后主动脉壁各层的屈服形状改变比能。

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Treatment of huge lumbar intervertebral disc herniation with ozone and collagenase through the route of inner margin of facet joint

小关节内侧缘入路注射臭氧与胶原酶治疗巨大型腰椎间盘突出症

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Methods Nucleus pulposus tissue taken from a one-month-old rabbit was treated by Trypsin and collagenase, and then the cells were cultured in DMEM/ F12 medium.

方法取一月龄新西兰兔的髓核，胰酶和胶原酶消化，DMEM/F12培养基中培养，倒置显微镜观察细胞形态。

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Sequestered Lumbar Disk Herniation: A Treatment with Collagenase Injection

胶原酶治疗破裂脱出型腰椎间盘突出症

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The immunizing conjugation product of collagenase in this program was prepared by EDCI method.

冷冻干燥法制备注射用胶原蛋白酶免疫结合物.

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Methods The thymic epithelial cells of systemic lupus erythematosus model ( BXSB mice) were cultured with merely shearing, shearing and collagenase digestion, shearing and trypsinization in different nutrient media.

方法：分别采用剪切法、剪切加胶原酶消化法、剪切加胰蛋白酶消化法建立培养体系获取自发性系统性红斑狼疮小鼠（BXSB小鼠）胸腺上皮细胞；

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Objective : To conclude therapeutic effect of systemic health education on intervertebral disclysis patients by collagenase.

目的: 总结运用系统健康教育程序对胶原酶溶盘术患者的效果.

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to investigate the mechanism of collagenase in preventing adhesion of intrathecal tendon.

研究胶原酶预防肌腱鞘内粘连的作用.

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The study of interstitial collagenase gene expression in esophageal carcinoma

食管癌间质胶原酶基因表达的研究

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The Study of Single-chain Fv Fragment Directed Against Type ⅳ Collagenase and Its Fusion Protein

抗Ⅳ型胶原酶单链抗体及其融合蛋白的研究单链核糖体失活蛋白的核糖核酸酶活性

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Methods: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion.

方法：酶消化法分离培养新生大鼠颅盖骨成骨细胞；

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drugs: 2% sodium phenobarbital, 0. 5% procaine, 0. 9% natrium chloride, 2% sodium hyaluronate, collagenase.

实验所需药品：2%苯巴比妥钠、0.5%普鲁卡因、0.9%氯化钠注射液、2%透明质酸钠、胶原酶。

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Conclusion The Neuroelectricity physiologic function of dorsal root ganglion and nerve would not be damaged by collagenase used in therapeutic concentration. ( J Intervent Radiol, 2006,15: 364-366)

结论临床应用的胶原酶化学髓核溶解术的胶原酶治疗浓度不影响大鼠脊神经背根神经节和传导神经的神经电生理功能。

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conclusion the collagenase with high quantity and activity could be induced in the improved medium.

结论通过改良并优化溶组织梭菌培养条件,可以获得低成本、高产量、高活性的胶原酶.

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