1、

Methods Nucleus pulposus tissue taken from a one-month-old rabbit was treated by Trypsin and collagenase, and then the cells were cultured in DMEM/ F12 medium.

方法取一月龄新西兰兔的髓核,胰酶和胶原酶消化,DMEM/F12培养基中培养,倒置显微镜观察细胞形态。

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Methods Chondrocytes were isolated from articular cartilage of 2-week-old rabbit, digested by collagenase ⅱ, and mixed with bovine collagen I, human blood freeze-dry fibrinogen and thrombosin according to a certain proportion, and were cultured in vitro.

方法:取2周龄的新生兔关节软骨,经消化,将获得的软骨细胞与牛型胶原、人血冻干纤维蛋白原、凝血酶按一定比例混合,制成软骨培养物并在体外培养。

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Treatment of huge lumbar intervertebral disc herniation with ozone and collagenase through the route of inner margin of facet joint

小关节内侧缘入路注射臭氧与胶原酶治疗巨大型腰椎间盘突出症

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4、

Conclusion Cutting and collagenase digestion process was easy, to obtain thymic epithelial cells of BXSB mice. We established a stable thymic epithelial cells culture system of systemic lupus erythematosus mice.

结论剪切、胶原酶消化法简便易行,可获得符合实验要求的BXSB小鼠胸腺上皮细胞,建立了稳定的系统性红斑狼疮模型鼠胸腺上皮细胞培养体系。

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Methods The thymic epithelial cells of systemic lupus erythematosus model ( BXSB mice) were cultured with merely shearing, shearing and collagenase digestion, shearing and trypsinization in different nutrient media.

方法:分别采用剪切法、剪切加胶原酶消化法、剪切加胰蛋白酶消化法建立培养体系获取自发性系统性红斑狼疮小鼠(BXSB小鼠)胸腺上皮细胞;

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Multipotent adipose-derived MSCs were isolated from subcutaneous, ovarian, or circumrenal adipose tissue of rabbit according to our method. Briefly, minced adipose tissue were digested with 0.1% collagenase type I and 1% BSA for 90 min at 37 ℃ with intermittent shaking.

首次采用O.1%胶原酶型I+1%BSA,消化90min的分离方法,从家兔腹股沟皮下脂肪组织,卵巢脂肪组织,或肾脏周围脂肪组织中分离得到脂肪MSCs。

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The study of interstitial collagenase gene expression in esophageal carcinoma

食管癌间质胶原酶基因表达的研究

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The influence on the numerical. count of Schwann cell ( SCs) from sciatic nerve and spinal ganglion was compared among various methods of enzymatic digestion technique, as homogenization or not and different enzymes ( trypsin, collagenase or a mixture of them) with various concentrations.

作者用酶消化的方法分离雪旺细胞(Schwann Cell,SCs),比较了匀浆与否,及不同浓度的胰酶、胶原酶、胰酶/胶原酶混合液对不同材料所获分离SCs数量的影响。

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Conclusion The Neuroelectricity physiologic function of dorsal root ganglion and nerve would not be damaged by collagenase used in therapeutic concentration. ( J Intervent Radiol, 2006,15: 364-366)

结论临床应用的胶原酶化学髓核溶解术的胶原酶治疗浓度不影响大鼠脊神经背根神经节和传导神经的神经电生理功能。

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effects of diammonium glycyrrhizinate on the lipid peroxidation and interstitial collagenase activity in fibrotic liver in rats

甘草酸二铵对肝纤维化大鼠肝脏脂质过氧化与间质性胶原酶活性的影响

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drugs: 2% sodium phenobarbital, 0. 5% procaine, 0. 9% natrium chloride, 2% sodium hyaluronate, collagenase.

实验所需药品:2%苯巴比妥钠、0.5%普鲁卡因、0.9%氯化钠注射液、2%透明质酸钠、胶原酶。

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The Study of Single-chain Fv Fragment Directed Against Type ⅳ Collagenase and Its Fusion Protein

抗Ⅳ型胶原酶单链抗体及其融合蛋白的研究单链核糖体失活蛋白的核糖核酸酶活性

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Methods: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion.

方法:酶消化法分离培养新生大鼠颅盖骨成骨细胞;

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Results The cementum on root surface showed synchronously-happened destruction after combined attack by acid and collagenase.

结果经酸性胶液和胶原酶联合攻击的根面牙骨质呈现脱矿与基质胶原纤维破坏同步发生的超微结构表现。

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Sequestered Lumbar Disk Herniation: A Treatment with Collagenase Injection

胶原酶治疗破裂脱出型腰椎间盘突出症

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Methods Rat model of hypertension with cerebral hemorrhage was induced by collagenase method.

方法采用胶原酶法制备高血压脑出血模型。

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Methods: 50 cases were treated by peridural injection of collagenase together with intravenous drip of red sage, which was compared with 25 cases of control group treated by red sage injection.

方法:用硬膜外注射胶原酶配合丹参静脉滴注治疗50例,与对照组丹参注射液治疗25例对照。

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Study on CT-guided collagenase injection in the treatment of herniation of L4-5 lumbar disc by posterolateral approach

CT引导下经旁路联合注射胶原酶治疗L4-5椎间盘突出症的临床研究

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19、

Objective: To investigate the effectiveness of CT guided collagenase injection into nucleus pulposus of herniated lumbar disc and the surface of herniation in the treatment of lumbar disc herniation.

目的:探讨CT引导下椎间盘突出的髓核内和突出物表面注射胶原酶治疗椎间盘突出症的效果。

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