2、

To induce intestinal venous congestion reperfusion model, portal vein was clamped for 45 min, then unclamped for 60 min. This reperfusion model was made in CR group and in Lipo-PGE1 group.

CR组和Lipo-PGE1治疗组均制作肠道急性淤血再灌注模型。模型制作:先阻断门静脉45min,再恢复血流60min。

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3、

Methods: All rats were subjected to openning chest then the left anterior desending coronary arteries ( LAD) were ligated for 30 min and unclamped for 60 min, measure the apoptotic cells by TUNEL, and detect the Caspase-3 by immunohistochemistry.

方法:采用在体大鼠结扎冠状动脉前降支30min后,松扎再灌注60min造成心肌缺血再灌注模型,使用TUNEL法进行细胞凋亡检测,使用免疫组化法测定凋亡相关蛋白Caspase3水平。

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