METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.

To integrate the results of clustering data mining and based on visual model requirement, network structure diagram of HQDS would be drawn, gene network structure be study with RT-PCR.

结合挖掘结果，运用网络结构可视化思想构建心气虚证网络结构图，并运用逆转录聚合酶链反应（RT-PCR）探索心气虚证基因网络结构。

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The mRNA amount of calcium regulatory proteins genes was measured by reverse transcription polymerase chain reaction and normalized to the mRNA levels of GAPDH.

通过逆转录聚合酶链反应技术，以GAPDH为内参照，测量SR的钙调控蛋白mRNA表达量。

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And semi-quantification reverse transcriptase-polymerase chase reaction ( RT-PCR) was used to analyse the effects of CIM and BET on mRNA expression of histamine receptors in the tissue of hepatocarcinoma.

并以逆转录聚合酶链反应（RT-PCR）半定量法研究BET、CIM对肝癌中组胺受体mRNA表达的影响。

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Serum myocardial enzymes was determined by biochemical method and the expression of c-fos mRNA in myocardium was detected by using reverse transcription polymerase chain reaction ( RT-PCR).

用生化方法检测血清心肌酶活性，逆转录聚合酶链反应（RT-PCR）方法检测心肌中c-fos基因的表达。

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Objective: To develop a sensitive, specific and accurate method ( reverse transcription polymerase chain reaction enzyme linked sandwich hybridization) for quantitative detection of human FGF mRNA.

目的：建立一种灵敏、特异、精确的定量检测人成纤维细胞生长因子mRNA（FGF mRNA）的逆转录聚合酶链反应酶联免疫吸附分析技术。

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The standard testing technique for Ebola is known as reverse transcription polymerase chain reaction, or RT-PCR.

标准的埃博拉检测技术被称作逆转录聚合酶链反应，简称RT-PCR。

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The RNA of islets in three purity was extracted, amplified with RT-PCR, and the mRNA expression of stem cell markers CK-19 and PDX-1 was detected.

分别将上述3组纯化后的胰岛抽提RNA行逆转录聚合酶链反应（RT-PCR），分析胰岛干细胞标志物CK-19及PDX-1mRNA的表达。

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METHODS: A sensitive nested-RT-PCR system was set up to make relative quantitative values of AFP mRNA of hepatic tissues and peripheral blood.

方法：建立敏感的巢式逆转录聚合酶链反应体系（Nested RT PCR），对肝组织和外周血中的AFP mRNA进行相对定量值分析。

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The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction ( RT-PCR) and Western blot.

采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。

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The expression of epidermal growth factor receptor was detected in the keratinocytes with immunocytochemistry and the transcription of epidermal growth factor receptor mRNA was determined with reverse transcription-polymerase chain reaction ( RT-PCR).

采用免疫细胞化学的方法检测表皮生长因子受体的表达，采用复合逆转录聚合酶链反应检测角质形成细胞中表皮生长因子受体mRNA的表达。

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Method The cell line was established by liquid cell culture.

方法液体培养法建立细胞系，用R显带和逆转录聚合酶链反应方法检测其遗传学标志；瑞氏染色、超微结构及组织化学染色观察细胞形态；

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TF and PAI-1 mRNA was detected by reverse transcription-polymerase chain reaction.

逆转录聚合酶链反应测定脂肪细胞TF和PAI1信使核糖核酸（mRNA）表达。

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With immunohistochemistry and reverse transcription poly-merase chain reaction, expressions of VEGF proteins and their mRNA in the ovarian tissue of each group were detected.

采用免疫组织化学法和逆转录聚合酶链反应技术检测卵巢组织VEGF蛋白质和mRNA的表达。

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Methods: The mRNA was extracted from the fresh tissues of 16 colonic cancer patients; reverse transcriptase polymerase chain reaction ( RT-PCR) was used to detect the receptors of TRAIL.

方法：取16例新鲜结肠癌组织提取mRNA，用逆转录聚合酶链反应（RT-PCR）方法检测TRAIL受体的表达。

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causes of unsteady running of engines

发动机运转不稳定的原因

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he moved his light horse toward haldon's mountains.

他将轻骑兵移向哈尔顿的山。

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lee's legion was commanded by henry 'light horse' lee, the father of famed confederate general robert e. lee.

李家军的指挥官是亨利·“轻骑兵”李，大名鼎鼎的邦联将军罗伯特·E·李的父亲。

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lightning fault analysis and the application of its locating system for transmission line

输电线路雷击故障分析及雷电定位系统应用

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application of occlusal adjustment in the preprosthetic orthodontic therapy in patients with periodontitis

调整咬合在牙周炎患者修复前正畸治疗中的应用

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the occlusal adjustment should be careful, accord to the request and prevent the new imbalance occlusion.

调牙合时要慎重，严格按照要求调磨，不要盲目操作，防止形成新的咬合不平衡。

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