The RNA of islets in three purity was extracted, amplified with RT-PCR, and the mRNA expression of stem cell markers CK-19 and PDX-1 was detected.

METHODS: A sensitive nested-RT-PCR system was set up to make relative quantitative values of AFP mRNA of hepatic tissues and peripheral blood.

方法：建立敏感的巢式逆转录聚合酶链反应体系（Nested RT PCR），对肝组织和外周血中的AFP mRNA进行相对定量值分析。

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The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction ( RT-PCR) and Western blot.

采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。

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The expression of epidermal growth factor receptor was detected in the keratinocytes with immunocytochemistry and the transcription of epidermal growth factor receptor mRNA was determined with reverse transcription-polymerase chain reaction ( RT-PCR).

采用免疫细胞化学的方法检测表皮生长因子受体的表达，采用复合逆转录聚合酶链反应检测角质形成细胞中表皮生长因子受体mRNA的表达。

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Method The cell line was established by liquid cell culture.

方法液体培养法建立细胞系，用R显带和逆转录聚合酶链反应方法检测其遗传学标志；瑞氏染色、超微结构及组织化学染色观察细胞形态；

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TF and PAI-1 mRNA was detected by reverse transcription-polymerase chain reaction.

逆转录聚合酶链反应测定脂肪细胞TF和PAI1信使核糖核酸（mRNA）表达。

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With immunohistochemistry and reverse transcription poly-merase chain reaction, expressions of VEGF proteins and their mRNA in the ovarian tissue of each group were detected.

采用免疫组织化学法和逆转录聚合酶链反应技术检测卵巢组织VEGF蛋白质和mRNA的表达。

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Methods: The mRNA was extracted from the fresh tissues of 16 colonic cancer patients; reverse transcriptase polymerase chain reaction ( RT-PCR) was used to detect the receptors of TRAIL.

方法：取16例新鲜结肠癌组织提取mRNA，用逆转录聚合酶链反应（RT-PCR）方法检测TRAIL受体的表达。

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causes of unsteady running of engines

发动机运转不稳定的原因

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he moved his light horse toward haldon's mountains.

他将轻骑兵移向哈尔顿的山。

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lee's legion was commanded by henry 'light horse' lee, the father of famed confederate general robert e. lee.

李家军的指挥官是亨利·“轻骑兵”李，大名鼎鼎的邦联将军罗伯特·E·李的父亲。

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lightning fault analysis and the application of its locating system for transmission line

输电线路雷击故障分析及雷电定位系统应用

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application of occlusal adjustment in the preprosthetic orthodontic therapy in patients with periodontitis

调整咬合在牙周炎患者修复前正畸治疗中的应用

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the occlusal adjustment should be careful, accord to the request and prevent the new imbalance occlusion.

调牙合时要慎重，严格按照要求调磨，不要盲目操作，防止形成新的咬合不平衡。

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Reversal of multidrug resistance and its mechanism by tetramethylpyrazine

川芎嗪逆转肿瘤多药耐药性及其机制的研究

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BACKGROUND OBJECTIVE: Multidrug resistance of tumor cells often leads to failure of chemotherapy. The over expression of P glycoprotein ( P gp), encoded by multidrug resistance 1 ( mdr1) gene, plays an important role in multidrug resistance of breast cancer.

背景与目的：肿瘤的多药耐药性常导致乳腺癌化疗失败，多药耐药基因1（Multidrug Resistance1，mdr1）编码的P-糖蛋白（P-glycoprotein，P-gp）过度表达是重要的耐药机制。

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Due to the complexity of tumor formation mechanism and the existence of multidrug resistance, it will not kill the tumor cells completely by using one drug alone.

但由于参与肿瘤形成的分子生物学机制的复杂性和多样性，以及肿瘤细胞多药耐药性的产生，使得仅仅利用一种化疗药物无法从根本上杀灭肿瘤细胞。

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Study on tumor cells' multidrug resistance and its reversion by Chinese herbs

肿瘤多药耐药性与中药逆转研究

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Progress in the study of drug delivery system based on nanoparticles to overcome multi-drug resistance

纳米粒给药系统逆转肿瘤多药耐药性的研究进展

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Study on Active Constituents of Traditional Chinese Medicine Reversing Multidrug Resistance of Tumor Cells in vitro

中药活性成分体外逆转肿瘤细胞多药耐药性的研究

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Conclusions: the multidrug resistance characteristic in SGC-7901/ 5-FU resistant cells may be related to micro-RNA expression profile changes, providing a new research direction to solve the multidrug resistance of tumor chemotherapy.

结论：SGC-7901/5-FU耐药细胞的多药耐药特性可能与micro-RNA表达谱的改变有关，为解决肿瘤化疗的多药耐药性提供新的研究方向。

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