transgene

Intraspecific Transplantation of Spermatogonial Stem Cells with Transgene

转基因精原干细胞异体移植

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Masculine transgene plant could be got by real time fluorescence PCR detection.

通过实时荧光PCR检测，获得阳性转基因植株。

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Study on the improving of wheat quality by pollen tube pathway transgene

花粉管通道法转基因改良小麦品质的初步研究

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【 Objective 】 To determine and compare the induction efficiency of RNA silencing triggered by different construction transgene of 3 ′-terminal segment derived from the Potato virus Y ( veinal necrosis strain, PVYN) coat protein gene.

目的探明马铃薯Y病毒脉坏死株系（PVYN）CP基因3′端序列短片段的不同结构转基因诱导转基因植物产生RNA介导抗性的有效性。

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Biphasic expression of c-myc transgene during hepatocarcinogenesis in WHV/ myc transgenic mice

WHV/myc转基因小鼠肝癌发生过程中c-myc基因表达的双相性

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We tested Morris water maze and found transgene mouse had a increased latent time in training phase and a decreased time in quadrantal of testing phase.

我们通过Morris水迷宫检测发现转基因小鼠的水迷宫训练阶段潜伏期延长，测试阶段在象限内时间较少。

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Whole mount in situ hybridization and transgene via microinjection are two important methods in zebrafish study.

整胚原位杂交和显微注射转基因技术是斑马鱼基因功能研究中两种重要的研究方法.

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Recently studies suggested that, such as DNA methylation and histone acetylation, could affect directly the cloning efficiency and transgene expression during reprogramming process of somatic cells.

已有的研究表明，体细胞重编程过程中，DNA甲基化、组蛋白乙酰化对克隆效率和转基因表达都有直接的影响。

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Current status and prospects of agricultural transgene technology in Sichuan province

四川省农业转基因技术重点领域及发展目标

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With the development of genetic engineering, more and more people pay attention to transgene silencing.

随着转基因技术在各个领域的深入发展, 转基因沉默已引起越来越多的人关注.

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To establish a cell strain expressing the transgene product will take time for colony isolation, colony amplification and colony characterization.

各位，我想构建一个载体，然后转染到肿瘤细胞中，以期获得稳定表达目的基因的克隆。

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Preliminary Exploration of Alfalfa Transgene Technique via Pollen-tube Pathway

紫花苜蓿花粉管通道法转基因技术初探

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Expatiated the safety evaluate of food: the safety problem of transgene food, the food pollution from antibiotic, the remain of pesticide, the chemistry material, microorganism of pathogeny and the food process.

阐述了食品的安全性评估：转基因食品的安全性问题，抗生素、农药残留、化学物质对食品的污染，病原微生物对食品的污染，食品加工方法中的污染等。

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Conclusion AAV-mediated striatal transgene expression of TH, AADC and GCH is more effective for gene therapy on Parkinsonian rat.

结论AAV载体介导的TH、AADC和GCH三重基因纹状体内共表达对于帕金森病大鼠的基因治疗更为有效。

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Progress of Soybean Transgene

大豆转基因的研究进展

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In the course of commercialization plant gene engineering , transgene silencing is one of the obstacles.

转基因植物中转基因沉默已成为植物基因工程的一大障碍.

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Relationship between PVY CP Gene Induced Resistance and Transgene Integration and Resistance Transmission

马铃薯Y病毒CP基因介导抗性与转基因整合方式的关系及抗性传导

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Objective To establish the technical data of transgene and cryopreservation.

目的建立一系列转基因技术和冷冻胚胎技术的实验室数据库。

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It is important to adopt efficient strategies to overcome gene silencing and increase transgene expression level for optimizing transgenic technology.

因而，MAR序列作为一种很有效的基因表达顺式调控元件，具有良好增强效果，MAR序列构建的高效表达载体对于优化转基因技术有非常重要的意义。

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The sequencing result showed that the nucleotide acid sequences of RT-PCR products were the same as that of transgene analyzed by BLAST software.

将RT-PCR产物测序后，用BLAST软件分析序列可知，该序列为细菌几丁质酶基因核苷酸序列而不是植物几丁质酶基因的核苷酸序列。

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Study of Rapid Detection of Transgene Sequences in Cottonseed Oil Using PCR

转基因棉子油的快速PCR检测研究

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Nuclear matrix attachment regions and transgene expression in plants

基质结合区（MARs）与转基因植物的基因表达

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Interleukin-4 gene was transformed into Chinese cabbage via pollen-tube pathway. Resistant selection by PPT, PCR amplification and PCR-Southern hybridization were used to detect whether transgene was in transformed progenies.

通过花粉管通道法介导il-4基因转化大白菜，利用PPT抗性筛选、PCR扩增和PCR-Southern杂交技术检测转化后代。

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At present cultivates the first batch breast albuminate iron albuminate transgene cow entered the lactation period.

目前培育的首批人乳铁蛋白转基因奶牛已进入泌乳期.

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CONCLUSION: The effect of MAR on transgene expression is related to the host cells.

结论：MAR能在一定程度上提高稳定外源基因的表达水平；MAR的作用与表达宿主相关。

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At the present time, the majority chemical pesticides used to prevent cotton bollworm are harmful to human and environment, and there is controversy on the security of the transgene crops such as the Bt cotton.

目前棉铃虫防治中使用的大多数化学农药对环境和人类健康有害，而对于转基因作物如Bt抗虫棉的安全性也存在争议。

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Establishment of transposon transgene system in buffalo.

水牛转座子转基因技术方法的建立。

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Current Status in Spatio-Temporal Expression and the segregation of transgene in the progenies of the Transgenic Plants

转基因植物中外源基因时空动态表达及遗传分离的研究进展

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Influencing factors on maize transgene process mediated by Agrobacterium

农杆菌介导玉米转基因的影响因素研究

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Mechanism and Functional Analysis of a Tobacco MAR on Stability of Transgene Expression

烟草核基质结合序列在转基因遗传稳定性中的功能及机理分析

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