restriction site

The Kpn ⅰ restriction site was introduced into the both ends and a random linking peptide of 3 amino acids into 3 ′-end.

并在突变体片段两端引入KpnⅠ位点，3′端引入3个氨基酸大小随机连接肽的序列。

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Restriction enzyme assay, PCR and sequencing confirmed that the TK gene was obtained successfully. Only one Kpn ⅰ site and a transcription termination signal TTTTT N ( T) T were found in the gene.

酶切鉴定、PCR鉴定和测序结果表明，成功获得了TK基因，获得的TK基因内存在一个KpnⅠ酶切位点和编码区的早期转录终止信号TTTTTN（T）T。

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With biological software analysis, obtained cDNA sequence base composition, codon usage and restriction site data, as well as detailed theoretical protein molecular weight, isoelectric point and amino acid composition.

利用生物软件进行分析，获得cDNA序列碱基组成、密码子使用频率和酶切位点等详细数据以及蛋白质理论分子量、等电点和氨基酸组成等情况。

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The first restriction of the first factor is the distance between rescuer and the earthquake site;

第1因素的第1制约条件为救援者与现场的距离；

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To understand the basic theory and practice method on manufactory working site management, break through the restriction of manufactory management, implement the lean production theory and production maintenance management method, realize effective production plan and Kanban management.

了解生产现场管理的基本理论和实践方式，突破生产管理中的约束点，推行精益生产理念和全面生产维护管理方式，实现有效的生产计划规划和看板管理模式。

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