pcr

The ISOObp promoter of PNZIP gene was cloned by adaptor PCR method.

因此,我们利用接头PCR技术克隆了PNZIP基因的启动子.

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Together with molecular bio_techniques like microarray, real_time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics.

同微阵列等分子生物技术一起，同步PCR技术将会在功能基因解析和病害分子诊断等方面发挥重要作用。

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PCR were using to detect bla ( TEM ), bla ( SHV ) and bla ( CTX-M ) genes.

应用聚合酶链反应(PCR)方法扩增产ESBLs株的bla ( TEM ) 、 bla ( SHV ) 和bla ( CTXM ) 基因.

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The mutation was confirmed by allele specific PCR ( ASPCR ).

应用等位基因特异性 PCR 验证测序所发现的突变.

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Microplate PCR Hybridization Technique Used in the Detection of Herpes Virus Genitals Infection

PCR微板杂交检测生殖器疱疹病毒感染

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Molecular diagnosis of α-thalassemia by using the real time PCR combined with the dissociation curve analysis

实时荧光聚合酶链反应结合融解曲线分析在α-地中海贫血基因诊断中的价值

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Objective To develop a multiplex fluorescent real-time PCR method and typing technique of herpes simplex virus for providing a rapid tool with a high sensitivity and specificity for the diagnosis of genital herpes and HSV typing.

目的建立单纯疱疹病毒的荧光多重实时PCR检测及分型技术，为生殖器疱疹的诊断及病毒分型提供快捷、敏感、特异的方法。

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RT-PCR was used to determine GK and PEPCKgene expression ( corrected by � �-actin ) .

用RT -PCR 检测GK和 PEPCK mRNA表达水平的变化.

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A method for rapid molecular diagnosis of sweetpotato stem nematode based on duplex PCR with species specific primer was described.

Ⅱ型甘薯茎线虫特异引物SSsj，和一个通用引物AB28共同扩增，特异扩增片段为623bp。

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Results The structures of recombinant plasmids were comfinaed by PCR and sequencing.

结果重组体经PCR和测序证实准确连接.

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Methods Taking quantity PCR as control, IIF mediated by group-shared HSV glucoprotein monoclonal antibody was adopted to detected 94 patients clinically diagnosed as GH.

方法以定量PCR为对照，用HSV型共同性糖蛋白单克隆抗体为夹心的IIF法，检测了94例临床诊断为生殖器疱疹的患者皮疹中的HSV。

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EST-PCR and EST-SSR marker were developed in Chinese cabbage.

建立了白菜的EST -PCR 和EST-SSR标记.

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PA-1 is resistant to27 kinds of antibiotics, mid-sensitive to1 kinds of antibioticThe hemolysin gene fragment of Vibrio harveyi VH-1 is amplified by PCR.

菌株PA-1对中国海洋大学硕士研究生毕业论文27种抗生素有抗性，对1种抗生素中度敏感。

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Results CnA α, CnA � � could be detected by RT-PCR but no CnA � �.

结果RT -PCR 可检测到CnAα 、 CnAβ,未检测到CnAγ.

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RT-PCR was used to detect the eNOS mRNA and iNOS mRNA.

RT -PCR 分别检测内皮细胞eNOS、iNOS基因的相对表达量.

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Amplification of immobilization antigen from Ichthyophthirius multifiliis by degenerate PCR

简并PCR扩增小瓜虫抑动抗原基因的ORF

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The expressions of bcl-x, bax and eNOS were measured by SQ-RT-PCR technique.

采用半定量逆转录聚合酶链反应(SQ-RT-PCR)法检测内皮细胞bcl-x 、 bax以及 eNOS表达.

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Methods: Polymer-ase chain reaction ( PCR ) was used to determine the ACE genotype.

方法: 采用聚合酶链反应 ( PCR ) 方法检测ACE基因型.

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To develop a real-time PCR method for herpes simplex virus-2 ( HSV-2).

目的建立一种快速、特异、准确定量的单纯疱疹病毒2型（HSV-2）检测方法。

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Histamine-producing strains could be detected by PCR test or DNA probe technology.

利用PCR与DNA探针技术能够快速检测葡萄酒中的组胺产生菌.

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Methods: HSV-2 antigen and antibodies were detected FQ-PCR and ELISA assay in 140 patients with GH.

方法：对性病门诊140例有现症生殖器疱疹（GH）患者用FQ-PCR检测生殖器疱疹病毒II型（HSV-2）和ELISA方法进行HSV-2抗原和抗体（包括IgG和IgM）检测。

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Methods Polymerase chain reaction ( PCR ) connected with reverse dot blot ( RDB ) were performed.

方法采用多聚酶链反应 ( PCR ) 结合反向斑点杂交 ( RDB ) 技术.

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Expression of TGF � � 1 mRNA was evaluated by RT PCR.

RT?PCR方法测定肺组织内TGF?β1的表达.

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Objective: To compare multiplex fluorescent PCR with serum type-specific antibody detection in diagnosis of genital herpes and evaluate it's significance in diagnosis of genital herpes.

目的：比较荧光多重PCR和血清型特异性抗体测定在生殖器疱疹临床诊断中的应用价值及评价各自的优缺点。

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All these prove that the gene is the new discovered i-ag gene and the degenerate PCR is one of the useful tool for searching and discovering the other members of i-ag gene family.

证实了iagFJ9是一个新发现的抑动蛋白基因，用简并PCR技术去发现小瓜虫抑动蛋白基因家族新基因是可行的。

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Methods PCR was performed to amplify the AMG encoding region.

方法　采用PCR技术体外扩增AMG完整分泌肽编码区.

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Under the optimal conditions, the detection limit of Japanese encephalitis virus was 2.0 × 103 PFU/ mL, which was 2 orders of magnitude lower than that of immunochromatographic strip and similar to that obtained from RT-PCR.

在最佳检测条件下，日本乙型脑炎病毒的检测限为2.0×103PFU/mL，比胶体金免疫试纸条法低两个数量级，与RT-PCR法的基本类似。

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REP-PCR , AFLP technique can reveal the genetic differentiation properly.

AFLP和 REP -PCR 技术均能很好地反映菌株的遗传学差异.

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The expression of mbcl-2 a gene was verified by the use of fluorescent microscope and RT-PCR technique.

方法设计一对特异引物,以含mbcl -2 α基因的载体pORF-mbcl-2α为模板,PCR扩增 出mbcl-2α目的片段,克隆至真核表达载体pTracer-EF及 pcDNA3.0中.

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Multiplex RT-PCR for Detection of Classical Swine Fever Virus and Japanese Encephalitis Virus

猪瘟病毒与猪流行性乙型脑炎病毒复合RT-PCR检测方法的建立

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