pcr的例句大全

SAP model was induced by injection of 3% sodium taurocholic acid into the subcapsular of pancreas. Serum amylase amylase of 3h, 6h, and 12h with SAP was measured. Expressions of IL 1 β mRNA in lung were detected by semi quantitative RT PCR.

采用牛磺胆酸钠胰腺被膜下注射诱导大鼠SAP模型，分别于术后3、6、12小时检测血清淀粉酶，半定量RT PCR检测肺组织IL1βmRNA的表达。

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Population genetic structure of Coilia ectenes sampled from Yangtze River estuary revealed by RAPD-PCR and ISSR-PCR markers

利用RAPD-PCR与ISSR-PCR标记技术分析长江口刀鲚的群体遗传结构

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The influences of iNOS and NO changes on the double-strand DNA breaks ( DSB) were identified using ligation-mediated PCR.

通过连接反应介导的PCR（LM-PCR）方法，评价iNOS和NO水平变化对DNA双链断裂（DSB）的影响。

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Methods Antibiotic susceptibility was tested by the disk diffusion to screen out the strains that produced the ESBLs; conjugative transfer test, plasmid profile, PCR and DNA sequencing were used to detect the phenotype or genotype of STEC.

方法采用纸片琼脂扩散法筛选36株产志贺毒素大肠埃希菌产ESBLs菌株，接合传递实验、质粒谱分析、PCR检测耐药基因和DNA序列分析产ESBLs菌株的表型和基因型。

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The positive protease can confirm this fungus and Aer gene with PCR can be measured to distinguish pathogenic and at present AH is included in the test scope for the pathogen of diarrhea.

蛋白酶阳性可确定该菌为致病性嗜水气单胞菌，另外还可用PCR检测Aer基因来区别致病性嗜水气单胞菌和非致病性嗜水气单胞菌。

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Detection of trans-genes resistant to glyphosate in soybean by combining test strip and PCR methods

试纸条法和PCR法检测抗草甘膦转基因大豆的外源基因

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To establish the polymerase chain reaction ( PCR) methed for detecting Moraxella catarrhalis with DNase testas reference and determine minimum inhibitory concentration ( MIC) of antimicrobial agents against M. catarrhalis.

目的：以DNA酶试验鉴定结果为标准，建立聚合酶链反应（PCR）鉴定卡他莫拉菌的方法，并测定抗菌药物对卡他莫拉菌的最低抑菌浓度（MIC）。

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The formation of calcium nodules were confirmed by Alizarin red S and tetracycline fluorescence staining. The expression of bone gla protein mRNA was examined by RT-PCR.

茜素红S、四环素荧光标记钙结节形成情况。RT-PCR检测骨钙素mRNA的表达。

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Results By asymmetric PCR, half of shadow bands of dinucleotide repeats were eliminated and there was only one band for each allele for tetranucleotide sequence.

结果利用不对称PCR，某些二核苷酸（CA）n重复序列的影子带减少一半，四核苷酸重复序列的影子带消除；

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A polymorphic tetranucleotide short tandem repeat ( STR) sequence in intron 3 of the phenylalanine hydroxylase ( PAH) gene was analysed in 52 normal individuals and 23 Phenylketonuria ( PKU) families by PCR amplification and silver staining.

应用PCR结合银染显色法分析了苯丙氨酸羟化酶基因内含子3中1个四核苷酸（TCTA）重复序列的多态性。

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Then various concentrations of polysaccharides were applied to different steps of virus replication cycle. Cytopathic effect ( CPE), methyl thiazolyl tetrazolium ( MTT) method and real time quantitative PCR were used as evaluation criterion.

选择不同剂量的刺参粘多糖作用于病毒增殖周期的多个环节，通过观察细胞病变（CPE）、MTT法检测病毒感染后活细胞存量、实时定量PCR检测HSV-1DNA作为评价指标；

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Conclusions It suggests that the PCR-RDB assay is effective in preventing the birth of β-thalassemia major and has clinical significance in improving the population quality.

结论PCR-RDB技术可有效减少β地中海贫血重症患儿的出生，具有重要的优生学意义。

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Analysis and Implementation of PCR Jitter Correction in Digital Television Transmission System

数字电视传输系统中PCR抖动的校正分析与实现

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Based on the standard of MPEG-2, the cause of PCR jitter and PCR correction method in Digital Television Transmission System are analyzed.

在MPEG-2标准的基础上，分析了PCR在数字电视传输系统中产生抖动的原因和校正的方法。

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The hardware implementation of PCR correction scheme in Digital Television Transmission System based on DMB-T is realized. Finally, the effect of PCR correction is given through analyzing the data collected in practical system.

同时在基于DMB-T的数字电视传输系统中用硬件实现了PCR校正单元，并通过对实际采集到的数据的分析，给出了PCR校正的效果。

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PCR-RFLP method was adopted to examine the genetic variety of genes ESR and FSH β in 5 pig populations ( Landrace and Large White from the farm of Zhenghong; Landrace, Large White, Duroc from the farm in the agriculture institute of Yiyang).

本研究采用PCR-RFLP方法检测了5个猪群体（正虹种猪场长白、大白；益阳农科所种猪场杜洛克、长白、大白）ESR和FSHP基因的遗传变异。

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For PCR amplification, template amount, DNA polymerase, annealing temperature and number of cycles might all affect the results of SNPs genotyping.

就PCR扩增来说，模板量、DNA聚合酶、退火温度、循环数都可能会影响聚丙烯酰胺凝胶电泳对SNPs的分型。

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Optimization of ISSR-PCR System for Lucuma nervosa by Using Orthogonal Design Through the study on the added amount of NaOH, reaction time and temperature, the optimum production process conditions were determined.

蛋黄果ISSR-PCR反应最佳体系的构建（简报）通过对氢氧化钠的加入量、应时间、应温度等生产条件的研究，得出了最佳生产工艺条件。

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The fabrication of the temperature control section of PCR-chip is very important.

PCR芯片的温度控制部分的搭构是决定整个PCR芯片性能、尺寸、集成性的重要组成部分。

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PCR-chip is a function biochip for DNA polymerase chain reaction. The temperature control section is so important that it decides the performance, dimension, integration of the whole chip.

PCR芯片是进行DNA聚合酶链式反应的芯片，温控部分的搭构是决定整个PCR芯片性能、尺寸、集成化的重要组成部分。

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