lysis

Methods Alkaline lysis assay was used to extract and purify the plasmid. Plasmid DNA was injected into tumor by microinjection assay.

方法：碱裂解法提取纯化质粒DNA，微量注射法直接将质粒DNA导入体内肿瘤；

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One inclusion was formed within the exosporium and remained with the spore after mother cell lysis.

电镜结果显示该晶体在芽胞外壁内侧形成,成熟后被包裹在芽胞外壁以内不脱落.

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After reproduction of the viral nucleic acid the host cell usually undergoes lysis ( LYSOGENY ).

病毒核酸复制后寄主细胞通常要经历裂解过程 ( 溶源状态 ).

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Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography.

方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。

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Finally, alkaline lysis and anion exchange chromatography were used to release and purify plasmids.

最后用碱裂解法裂解细胞提取质粒，并将提取物进行阴离子交换层析以进一步纯化质粒。

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The plasmids were extracted by alkaline lysis technique.

应用碱裂解法提取相应菌株的质粒，对质粒谱进行分型。

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After such pre-treatments, the materials were denatured by a modified alkaline lysis procedure and their mtDNA was extracted.

经过处理后的材料分别再用改良的碱变性法[2～6]提取mtDNA，并比较相应处理的mtDNA。

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Hyperuricemia is associated with and progression of gout, cardiovascular diseases, tumor lysis syndrome and renal disease.

高尿酸血症与痛风 、 心血管疾病 、 肿瘤裂解综合征及肾脏疾病的引发或加剧密切相关.

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This leads to the loss of osmotic balance and ATP, and finally to cell lysis.

这会导致渗透不平衡和atp的散失, 并最终导致细胞的凋亡.

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Conclusion Scar excision and lysis with constant traction might be an optimal method in the management of severe postburn scar contracture deformity of joints in the lower extremities and satisfactory results could be obtained without tendon grafting and elongation.

结论严重关节挛缩畸形的患者采用瘢痕切开松解、持续牵引的方法治疗，无需行肌腱移植与延长术，功能恢复良好。

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Close lysis for the treatment of tenovaginitis of flexor digitorum

闭合松解术治疗手指屈指肌腱腱鞘炎

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Thirty genomic DNA of Fusarium were extracted with improved SDS alkaline lysis method and the ITS district of rDNA was especially amplified.

实验运用改良的SDS碱裂解法对30株镰刀菌基因组DNA进行了抽提，并对rDNA ITS区域进行了特异性扩增。

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As the final lysis of his disease, his skin returned to its original color.

当他的疾病终于渐退, 他的皮肤恢复为原本的颜色.

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Objective : To explore the risk factors and treatment of tumor lysis syndrome ( TLS ) .

目的: 探讨肿瘤溶解 综合征 发生的危险因素及其治疗.

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The relationships between efficiencies and conditions of cell lysis were also studied. Through environmental scanning electron microscope ( SEM), the effects of ultrasound, acid, alkaline and thermal action on the microorganism shape in sludge were analysised.

通过环境扫描电镜（SEM）技术分析了超声波、酸、碱以及热作用对污泥中微生物的形态影响以及各种预处理方法对污泥溶胞效果的影响关系。

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The final yield of plasmid DNA from the broth after alkaline lysis is 56.61%, and its purity determined by agarose electrophoresis is almost 100%.

结果表明：以碱裂液中质粒DNA为计算基准的得率为56.61%、超螺旋质粒DNA的电泳纯度100%。

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Objective To determine the optimal condition of lysis of influenza virus by sodium deoxycholate.

目的研究去氧胆酸钠对流感病毒最佳的裂解条件.

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The procedure of Southern hybridization has been improved as follows: the plasmid DNA which contains DNA fragments for probe was isolated by alkaline lysis without utilizing cesium chloride gradient centrifugation.

对传统的Southern杂交法进行了改进：用碱变性和酚抽提法提取重组质粒，省去氯化铯梯度离心；

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At high calcium concentration they fuse without lysis.

在钙离子浓度高时,他们融合而不解体.

词典精选例句

The effect of ultrasonic irradiation with energy density of 0.05 W · mL-1 and thermal action on activated sludge cell lysis under pH ranging from 3.0 to 12.0 has been studied experimentally in detail.

在能量密度为0.05W·mL-1和pH范围3.0～12.0条件下，研究了超声波辐射以及热作用对污泥溶胞效果的影响，考察剩余污泥的溶胞效果与溶胞条件的关系。

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Conclusion Fibroblasts are related to conponent loosening and bone lysis.

结论这提示Fb对假体松动及骨溶解有影响。

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Preliminary Study on Condition for Lysis of Human Respiratory Syncytial Virus

人呼吸道合胞病毒裂解条件的初步探索

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Objective to explore the action of main reagent alkaline lysis method of extracting plasmid DNA.

目的对碱裂解法提取质粒DNA中主要试剂的作用进行研究。

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In this paper the authors described the principle of rehabilitation after tendon lysis, tendon anastomosis, tendon grafting and severed hand rejoining.

本文主要阐述了肌腱松解术、肌腱吻合术、肌腱移位术和断手再植术后的康复原则。

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Use alkaline lysis method to extract the plasmid DNA. DNA constructs were identified by Pst I/ Bgl II digestion, denaturing polyacrylamide gel electrophoresis and DNA sequence analysis.

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

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Hence, a successful industry ana-lysis will have to identify the underlying fac-tors driving demand and supply.

因此, 成功的行业分析,便须对那些影响供需的基本因素作出辨别.

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The lysis of the brain takes place only hours after death.

脑的细胞溶解,在死后的几小时就发生了.

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Testing of Capacitor Voltage Transformer and Arrester without Removal of the Lead The practical suture lysis methods for high intraocular pressure after trabeculectomy

500kV电容式电压互感器、避雷器不拆线试验方法青光眼小梁切除术后高眼压拆线方法降眼压的探讨

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There are three main methods , drop collapse , oil spreading , and blood lysis, to detect biosurfactant production.

生物表面活性剂的检测方法主要有:液滴坍塌法 、 排油圈法和血平板法.

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However, the recovery of recombinant proteins hinges largely on the ability to effectively induce cell lysis.

然而, 重组蛋白的提取有赖于诱导细胞溶解的能力.

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