Ten animals ( 1 rat from each of the 10 litters) in each group were killed on the 10th and 21st day after birth respectively. Hippocampi were removed for determination of proenkephalin mRNA expression by RT-PCR.

Reverse transcription-polymerase chain reaction ( RT-PCR) was developed for the detection of dengue virus 3 genome.

本文应用逆转录聚合酶链反应（RT-PCR）方法，检测Ⅲ型登革病毒基因。

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The α 3 and α 7 nAChR subunits in protein level were measured by Western blotting and in mRNA level by RT-polymerase chain reaction ( RT-PCR).

用蛋白印记方法测定α3和α7尼古丁受体亚单位蛋白水平；用逆转录聚合酶链反应方法测定尼古丁受体亚单位mRNA水平。

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Methods: The blood samples were collected from HFRS patients between 2000~ 2001, and the M segment genome of hantavirus was amplified by RT-PCR.

方法：采集2000~2001年肾综合征出血热（HFRS）患者的血液标本，通过逆转录聚合酶链反应（RT-PCR）扩增汉坦病毒M片断基因。

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Objective To establish the real-time RT-PCR method to detect the mRNA level of dihydropyrimidine dehydrogenase, and to determine the expression of dihydropyrimidine dehydrogenase in various human tissues in RNA level and protein level.

目的建立实时逆转录聚合酶链反应（RT PCR）检测二氢嘧啶脱氢酶mRNA的定量方法，并分别从RNA水平与蛋白质水平检测二氢嘧啶脱氢酶在人体部分组织中的表达。

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Using reverse transcription polymerase chain reaction ( RT-PCR), CCK-A receptor ( CCK-AR) and CCK-B receptor ( CCK-BR) mRNA were expressed in myocardium of rats.

由逆转录聚合酶链反应（RT PCR）检测到心肌组织有CCK A受体（CCK AR）和CCK B受体（CCK BR）mRNA表达。

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Detection mucin gene in cervical lymph nodes in supraglottic cancer by reverse transcription polymerase chain reaction

粘蛋白基因逆转录聚合酶链反应法检测声门上型喉癌N0颈淋巴结微转移

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Immunocytochemistry showed that there was an evident expression of epidermal growth factor receptor in the keratinocytes. The transcription of epidermal growth factor receptor mRNA was apparent according to RT-PCR.

免疫细胞化学显示表皮生长因子受体在细胞表面有明显的表达，复合逆转录聚合酶链反应显示表皮生长因子受体mRNA有明显的表达。

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METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.

我们用A549细胞研究PARs在肺上皮细胞的表达.方法：采用逆转录聚合酶链反应（RT-PCR）、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系A549细胞PARs的表达情况。

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To integrate the results of clustering data mining and based on visual model requirement, network structure diagram of HQDS would be drawn, gene network structure be study with RT-PCR.

结合挖掘结果，运用网络结构可视化思想构建心气虚证网络结构图，并运用逆转录聚合酶链反应（RT-PCR）探索心气虚证基因网络结构。

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The mRNA amount of calcium regulatory proteins genes was measured by reverse transcription polymerase chain reaction and normalized to the mRNA levels of GAPDH.

通过逆转录聚合酶链反应技术，以GAPDH为内参照，测量SR的钙调控蛋白mRNA表达量。

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And semi-quantification reverse transcriptase-polymerase chase reaction ( RT-PCR) was used to analyse the effects of CIM and BET on mRNA expression of histamine receptors in the tissue of hepatocarcinoma.

并以逆转录聚合酶链反应（RT-PCR）半定量法研究BET、CIM对肝癌中组胺受体mRNA表达的影响。

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Serum myocardial enzymes was determined by biochemical method and the expression of c-fos mRNA in myocardium was detected by using reverse transcription polymerase chain reaction ( RT-PCR).

用生化方法检测血清心肌酶活性，逆转录聚合酶链反应（RT-PCR）方法检测心肌中c-fos基因的表达。

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Objective: To develop a sensitive, specific and accurate method ( reverse transcription polymerase chain reaction enzyme linked sandwich hybridization) for quantitative detection of human FGF mRNA.

目的：建立一种灵敏、特异、精确的定量检测人成纤维细胞生长因子mRNA（FGF mRNA）的逆转录聚合酶链反应酶联免疫吸附分析技术。

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The standard testing technique for Ebola is known as reverse transcription polymerase chain reaction, or RT-PCR.

标准的埃博拉检测技术被称作逆转录聚合酶链反应，简称RT-PCR。

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The RNA of islets in three purity was extracted, amplified with RT-PCR, and the mRNA expression of stem cell markers CK-19 and PDX-1 was detected.

分别将上述3组纯化后的胰岛抽提RNA行逆转录聚合酶链反应（RT-PCR），分析胰岛干细胞标志物CK-19及PDX-1mRNA的表达。

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METHODS: A sensitive nested-RT-PCR system was set up to make relative quantitative values of AFP mRNA of hepatic tissues and peripheral blood.

方法：建立敏感的巢式逆转录聚合酶链反应体系（Nested RT PCR），对肝组织和外周血中的AFP mRNA进行相对定量值分析。

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The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction ( RT-PCR) and Western blot.

采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。

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The expression of epidermal growth factor receptor was detected in the keratinocytes with immunocytochemistry and the transcription of epidermal growth factor receptor mRNA was determined with reverse transcription-polymerase chain reaction ( RT-PCR).

采用免疫细胞化学的方法检测表皮生长因子受体的表达，采用复合逆转录聚合酶链反应检测角质形成细胞中表皮生长因子受体mRNA的表达。

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Method The cell line was established by liquid cell culture.

方法液体培养法建立细胞系，用R显带和逆转录聚合酶链反应方法检测其遗传学标志；瑞氏染色、超微结构及组织化学染色观察细胞形态；

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TF and PAI-1 mRNA was detected by reverse transcription-polymerase chain reaction.

逆转录聚合酶链反应测定脂肪细胞TF和PAI1信使核糖核酸（mRNA）表达。

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