Effect of Different Transfection Methods and Green Fluorescent Protein Expression Vectors on Transfection Efficiency in Mouse Embryonic Stem Cell

1、

Objective: To increase the efficiency of mouse embryonic stem cell ( ES) chimeras production through improving the culture condition to maintain undifferentiated state of ES cells.

目的：探讨如何通过改善小鼠胚胎干细胞（Embryonic stem cell，ES）的培养条件来维持其未分化状态，提高嵌合体的制备效率。

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2、

PURPOSE: To culture primary mouse odontoblast and to provide a base for study on inducing ES cells to odontoblast.

目的：原代培养小鼠成牙本质细胞，为诱导胚胎干细胞（ES细胞）向成牙本质细胞分化提供基础。

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3、

Teratoma formation was performed to analyze the differentiation status and potential of the GFP ES cells.

对稳定表达GFP的胚胎干细胞系进行畸胎瘤形成检测，观察其多向分化潜能。

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4、

Knockout serum replacement improves establishment efficiency of C57BL/ 6J mouse embryonic stem cell line

Knockout血清替代品可提高C57BL/6J小鼠胚胎干细胞建系效率

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5、

Objective To investigate the probabilities of embryonic stem ( ES) cells differentiating into neuronal cells in vitro.

目的探讨胚胎干细胞（ES）向神经细胞定向分化发育的可能性。

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6、

Objective To observe the effect of transplantation of embryonic stem cell ( ES) on neurological functional recovery of injured spinal cord in adult mouse.

目的观察胚胎干细胞（Embryonic stem cell，ES）诱导的神经前体细胞移植，对小鼠脊髓损伤神经功能恢复的影响。

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7、

The Presentation of neo Gene in Chimeric Mice Obtained from ES Cells

通过胚胎干细胞途径获得的嵌合体小鼠中neo基因在各组织中的分布

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8、

Meth-ods: Human embryonic stem cells were cultured in suspension without feeder layers and bFGF in medi-um.

方法：胚胎干细胞在无饲养细胞、无碱性成纤维细胞生长因子（bFGF）、悬浮条件下培养形成类胚体。

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9、

Differentiation of embryonic stem cell derived retinal precursor cell after transplanted into subretinal space in rd mouse

胚胎干细胞源性视网膜前体细胞在rd鼠视网膜下腔移植后的分化

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10、

Synchronization, Neuro-differentiation and Amyloid-β Peptides Inducing NF κ B Activation During Differentiation from Mouse Embryonic Stem Cells to Neuronal Cells in Vitro

胚胎干细胞同步化处理、神经分化及Aβ对分化神经元NFκB通路的影响

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11、

Establishment of cell line of mouse embryonic stem cells and its label with GFP

小鼠胚胎干细胞建系及GFP标记

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12、

The Effects of Mouse ES Cell Conditioned Medium on Isolation and Cloning of Sheep Embryonic Stem-like Cells

小鼠ES细胞条件培养基对绵羊类胚胎干细胞克隆、传代的影响

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13、

The immunophenotype and the pluripotent marker expression were obtained by flow cytometry analysis.

运用流式细胞技术检测MSC的免疫表型和胚胎干细胞的全能标志物。

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14、

Objective To investigate the possibility of commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats.

目的探讨视网膜神经细胞培养的上清液对胚胎干细胞（embryonic stem cells，ES）体外分化的诱导作用。

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15、

Influences of LIF and IGF-1 on the isolation of bovine ES cells

LIF和IGF-1对牛胚胎干细胞分离的影响

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16、

Methods: To optimize the basic cultivation conditions of ES cells;

方法：优化胚胎干细胞（ES细胞）的基本培养条件；

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17、

Effect of VEGF on Neural Differentiation of Human Embryonic Stem Cells in vitro

VEGF在人胚胎干细胞向神经元分化中作用的研究

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18、

Nucleus transplantation technology could make nuclear transfer embryonic stem cell lines. Using ES cells could make chimera animals and modify genes, etc.

可以利用核移植技术建立核移植胚胎干细胞（nuclear transfer embryonic stem cells.ntES）系，利用胚胎干细胞制备嵌合体动物，对胚胎干细胞进行基因改造等研究。

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Human Endostatin Gene Transfered into CD34+ Hematopoietic Stem Cells from Human Umbilical Cord Blood

Endostatin基因导入人脐带血CD34+造血干细胞的实验研究

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Background: leukemia is a malignant desease from the hemopoietic stem cell ( HSC), it seriously threatens the health and lives of human beings.

背景白血病（leukemia）是造血干细胞克隆性增殖的肿瘤性疾病，严重威胁人类健康和生命。

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