gene silencing

Gene silencing is a kind of epigenetic phenomenon discovered in transgenic plants in recent years.

摘要基因沉默是近几年来在转基因植物中发现的一种后生遗传现象.

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Genetic Diversity of Potato Spindle Tuber Viroid ( PSTVd) and Its Induced Gene Silencing

马铃薯纺锤块茎类病毒遗传多样性及其诱导的基因沉默

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DNA methylation catalyzed by DNA methyltransferases interacts with histone modification to inhibit gene transcription, induce gene silencing at the participation of many regulators.

DNA甲基化由DNA甲基转移酶催化，在多种调节因子的参与下，与组蛋白修饰相互作用，抑制基因转录，导致基因沉默。

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Present studies show that many different kinds of protein of plant virus which can inhibit the Post Transcriptional Gene Silencing of plants such as CMV-2b-pro and PVY-HC-pro etc have been found. The corresponding inhibitive mechanism of virus protein have been Understood.

目前研究发现黄瓜花叶病毒（CMV）的2b蛋白及马铃薯丫病毒（PVY）的HC蛋白等植物病毒蛋白能抑制植物转录后基因沉默，对它们相应的抑制机理已了解。

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Antisense RNA technology was an effective way to silencing specific gene, and was widely employed in plant breeding.

反义RNA技术是一种很有效的使基因沉默的方法，目前已广泛应用于植物的遗传育种中。

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Finally, the researchers showed that DNMT 1-stimulated HP 1 binding is required for gene silencing.

最后, 研究者指出DNMT1激发性Hp1蛋白的结合作用需要基因沉默.

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Stable expression systems can be efficiently delivered to mammalian cells by viral vectors, resulting in long-term silencing of the target gene.

另外，通过病毒载体构建的稳定表达体系可以有效的应用于哺乳动物细胞，使靶标基因长期沉默。

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This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handing nucleic acid.

这个RNA干扰的实用指南将使得任何具有基本的核酸处理设备的实验室具备使目标基因沉默的能力。

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Caused by the methylation of gene silencing the tumor, available through DNA methyltransferase non-competitive or competitive inhibitors methylation occurs, activating the silence of the tumor suppressor gene, so as to achieve the aim of the treatment of tumors.

由甲基化引起基因沉默而出现的肿瘤，可通过DNA甲基转移酶非竞争性或竞争性抑制剂抑制甲基化的发生，活化沉默的抑癌基因，从而达到治疗肿瘤的目的。

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Gene silencing by siRNAs has become a quick and efficient experimental method to study gene function and therapeutic intervention in the disease characterized by aberrant gene expression.

siRNAs介导的基因沉默已经成为一种快速有效的研究基因功能和对基因突变疾病进行干预的措施。

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RNAi ( RNA interference) is a conservative phenomenon and one of important mechanisms for post-transcriptional gene silencing in living nature.

RNA干扰（RNAi）是生物界中一种既古老且在进化上又高度保守的现象，是基因转录后沉默的重要机制之一。

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Distant hybridization of plants can cause a wide range of apparent genetic variability, such as: gene silencing, nucleolar dominance, transposon activation, DNA methylation variations and so on, and DNA methylation, in particular cytosine methylation is one of the main epigenetic control mechanisms.

植物远缘杂交可以引起广泛的表观遗传变异，如：基因沉默、核仁显性、转座子激活、DNA甲基化变异等，而DNA甲基化尤其是胞嘧啶甲基化是表观遗传的主要调控机制之一。

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It is important to adopt efficient strategies to overcome gene silencing and increase transgene expression level for optimizing transgenic technology.

因而，MAR序列作为一种很有效的基因表达顺式调控元件，具有良好增强效果，MAR序列构建的高效表达载体对于优化转基因技术有非常重要的意义。

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RNA interference, as an important gene silencing methods, it has a significantapplication to the research of gene function and genetic melioration.

RNA干涉是一种重要的基因沉默方式，它在基因功能和遗传改良研究中有重要的应用。

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Gene silencing can be divided into two types: position effect and homology dependent gene silencing ( HdGS ) .

基因沉默大体可以分为两类: 位置效应引起的基因沉默和同源依赖的 基因沉默.

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Gene silencing is also concerned with the growth of the organism.

同时,基因沉默还与生物的生长发育有关.

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In vertebrates, long dsRNA activates the interferon response and yield non-specific degradation of mRNA. In contrast, small interference RNA ( siRNA) duplexes with the length of 21 to 23 nucleotides trigger specific gene silencing and thus be widely used for gene functional studies.

脊椎动物中，长链dsRNA能引发干扰素反应而引起非特异性的mRNA降解，而21-23nt长度的siRNA能特异性介导基因的沉默，因而被广泛应用于基因功能研究。

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MTHFR gene silencing affects mouse embryonic palatal mesenchymal cell proliferation and apoptosis

MTHFR基因沉默对小鼠胚胎腭突间充质细胞增殖和凋亡的影响

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RNA interference ( RNAi), an evolutionary highly conserved process of posttranscriptional gene silencing, can be triggered by small interfering RNAs ( siRNAs) that mediate sequence-specific mRNA degradation.

RNA干扰（RNAi）是指生物体内由小干扰RNA（siRNAs）介导的具有高度特异性，只降解与之序列相应的单个内源基因mRNA的，在进化上高度保守的转录后基因沉默现象。

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The review summarized the application and perspective of reverse genetics technique in many aspects such as reviving virus, creating new virus, researching negative RNA virus and function of animals and plants gene, using RNA interference for gene silencing.

文章从拯救病毒和创造新型病毒、负股RNA病毒的研究、动植物基因功能的研究以及利用RNA干扰使基因沉默等方面，对反向遗传学技术的应用进行了评述，并对其前景进行了阐述。

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Gene silencing using small interfering RNA ( siRNA) is a potent method of specifically knocking down molecular targets.

使用小干扰RNA（siRNA）特异性的敲除分子靶点是一种新兴的基因沉默的方法。

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Fruit-specific RNAi-mediated Lcy Gene Silencing Enhances Content of Lycopene in Tomatoes

果实特异性RNAi介导的Lcy基因沉默来增加番茄中番茄红素的含量

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Small RNAs can guide posttranscriptional degradation of complementary messenger RNAs and, in plants, transcriptional gene silencing by methylation of homologous DNA sequences.

小RNA能诱导互补信使RNA在转录后降解，对于植物，可通过同源DNA序列甲基化使转录基因沉默。

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