blastocoele

Methods We isolated embryonic stem cells from inner cell mass of KM mice blastocysts. Then we transferred embryonic stem cells into blastocoele of C57BL/ 6J inbred mice by microinjection in order to construct chimeric mice.

方法从KM小鼠囊胚的内细胞团中分离培养胚胎干细胞，进行近交系C57BL6J小鼠受体囊胚腔内注射，进行嵌合体构建实验。

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the time-lapse recording showed that at this stage the blastocoele collapsed at least once in 25 of the 26 embryos (96%).

实时监控录像显示在此阶段26枚胚胎中有25枚（96%）至少发生一次囊胚腔萎缩。

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The time-lapse recording showed that at this stage the blastocoele collapsed at least once in25 of the26 embryos ( 96%).

实时监控录像显示在此阶段26枚胚胎中有25枚（96%）至少发生一次囊胚腔萎缩。

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After thawing, 26 of the33 embryos ( most of which were composed of between two and ten cells) developed to blastocyst stage in which the blastocoele is formed.

解冻后，33枚胚胎中有26枚（大多数胚胎含有2到10个细胞）发育到囊胚阶段，此时囊胚腔已经形成。

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