auxotrophic

We obtained the auxotrophic mutantS of k ( lys, p) and Q ( arg-) by mutation with DES. The frequency of fusion is 1. 25 per 105 protoplast.

通过硫酸二乙酯（DES）诱变得到营养缺隐型菌株Q（arg－）和K（lys－，ρ－），其融合率为1.25×10－5。

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Auxotrophic Mutant Selection and Protoplast Fusion in Saccharomyces cerevisiae

啤酒酵母（saccharomyces cerevisiae）营养缺陷型的筛选及原生质体融合

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By using microcalorimetry method, we have studied ura auxotrophic mutant of saccharomyces cerevisiae AY, and 3 strains transformed from saccharomyces cerevisiae AY by plasmids.

用微量热法研究了尿嘧啶缺陷型酿酒酵母AY，和该菌株分别经穿梭质粒pYLZ-2、重组质粒pYLZ-2/f27、pYLZ-2/622转化的3种菌株的生长代谢热动力学。

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Prototrophic hybrids have been obtained by the protoplast fusion between auxotrophic haploid and diploid mutants of Saccharomyces cerevisiae.

本文采用营养缺陷型的互补进行了啤酒酵母原生质体融合产物的筛选。

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Compared ethanol fermentation power of these 18 fusants with 23 fusants selected by auxotrophic marker, the results demonstrated that percentage of high ethanol-yielding strains in fusants population selected by RAPD marker was significantly high than fusants population selected by auxotrophic marker.

该18株融合子酒精发酵能力经与传统的用营养缺陷型标记筛选的23株融合子比较，表明用RAPD标记筛选到的融合子群体中，高产菌株所占的比例明显高于前者。

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Nine auxotrophic mutant strains were obtained by using~ ( 60) Co-γ ray irradiating Auricularia basidiospores.

利用~（60）Co&γ射线辐照木耳担孢子，获得了9株营养缺陷型突变体。

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Mutagenesis of Auxotrophic Mutants of Penicillium chrysogenum by NTG

NTG诱发产黄青霉菌产生营养缺陷型突变体的研究报道

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Preparation of double mutants through crosses between compatible auxotrophic mutants in higher basidiomycetes

高等担子菌双重营养缺陷突变菌株的分离

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Mutagenic Effects of Laser on Auricularia auricula and Selection of Auxotrophic Mutant

黑木耳激光诱变及营养缺陷型突变体筛选

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Further investigation demonstrated that UV light decreased the mating ability of ade auxotrophic haploids and had no significant effect on the mating ability of other auxotrophic haploids.

研究表明：UV减弱了腺嘌呤缺陷型单倍体的交配能力，而对其它营养缺陷型单倍体的交配能力无甚影响；

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Selection of high α-amylase yeilding strains of Bacillus subtilis by auxotrophic reversion technique

应用营养缺陷型回复突变的方法筛选枯草杆菌（Bacillus subtilis）α-淀粉酶的高产菌株

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Auxotrophic mutants of Trichoderma harzianum tolerant to carbendazim were obtained by UV-light mutagenesis and carbendazim induction on PDA medium.

用紫外线诱变哈茨木霉野生型菌株，经含多菌灵PDA培养基耐药性筛选，获得了耐药性菌株。

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ADI is a potential cancer therapy agent for the treatment of arginine-auxotrophic tumors ( such as hepatocellular carcinomas and melanomas), and it widely exists in bacteria, achaea and some anaerobic eukarya.

ADI广泛存在于细菌、古细菌、厌氧真核生物中，它是一种治疗精氨酸缺陷型肿瘤（如肝细胞癌、黑素瘤）的药物。

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Induction and selection of auxotrophic mutants from the protoplasts of Lentinus edodes screening and characteristics of mutants of E. coli resistant to acetate inhibition

香菇原生质体诱变及营养缺陷型突变体筛选大肠杆菌乙酸代谢突变株的选育和特性研究

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The effect of volumetric oxygen transfer coefficient ( kLa), on the performance of producing pyruvate by a multi-vitamin-auxotrophic yeast of Torulopsis glabrata, was investigated in batch fermentation process.

以一株光滑球拟酵母的多重维生素营养缺陷型为研究菌株，考察了分批发酵中不同体积传氧系数（KLa）对其产丙酮酸性能的影响。

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Selection of the Optimum Conditions of Preparing an Auxotrophic Saccharomyces cerevisiae Protoplast by Orthogonal Tests

用正交实验法筛选营养缺陷型酿酒酵母原生质体的最佳实验条件

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Studies on the Regeneration Mode of the Protoplast of Auxotrophic Mutants of Auricularia

木耳营养缺陷型原生质体再生方式研究

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The optimal conditions for protoplast isolation and regeneration of4 auxotrophic mutant strains of Trichoderma were studied.

研究了4种营养缺陷型产纤维素酶菌的原生质体形成和再生条件。

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Then at the 6 point will lead to a higher pregnancy rate. The reverse mutation frequency of the protoplast of the auxotrophic mutants was less than 10 ￣(-6).

营养缺陷型菌株原生质体回复突变率小于10￣（－6）。

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