agarose gel

METHODS: The DNA content and percentage of apoptosis were measured by flow cytometry; Morphological changes were observed with light microscope and electron microscopy; DNA fragmentation was analyzed by agarose gel electrophoresis and terminal dUTP nick end labeling ( TUNEL) method.

方法：用流式细胞术检测DNA含量及凋亡细胞百分率，透射电镜观察细胞形态学变化，DNA琼脂糖凝胶电泳和原位末段标记（TUNEL）检测DNA断裂。

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Haemoglobin polymorphism of175 thoroughbred foals in China was examined by agarose gel isoelectric focusing.

采用等电聚焦电泳对175匹中国纯血马幼驹进行了血红蛋白多态性的检测。

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Agarose gel electrophoresis was used to terrify the specificity of the PCR reacted products. Results: 1.

使用琼脂糖凝胶电泳检测PCR反应产物的特异性。

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A ladder-like pattern of DNA fragmentation appeared upon agarose gel electrophoresis.

琼脂糖凝胶电泳可见“梯状”现象.

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Agarose gel electrophoresis of the DNA extrate from cells treated with ActD shows typical DNA ladder.

DNA琼脂凝胶电泳呈典型的凋亡细胞DNA梯形条带；

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The PCR products were electrophoresed on 3 % agarose gel and visualized under UV light.

聚合酶链锁反应的产物以3%琼脂电泳分离并在紫外光下观察结果.

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Methods: Lyse the blood cells with NaI, extract DNA with Chloroform-Isopentanol, precipitate DNA with alcohol, determine concentration and purity of the DNA with UV spectrophotometry and agarose gel electrophoresis.

方法，使用碘化钠裂解血细胞，氯仿-异戊醇抽提DNA，乙醇沉淀DNA，紫外分光光度法和琼脂糖凝胶电泳测定DNA的浓度和纯度。

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DNA extract of four groups was examined by agarose gel electrophoresis, the results showed that: DNA band was bright, clear, tidiness and non-degeneration phenomenon. There was no DNA band appeared in negative control group.

4组样品的DNA提取物经1.5%琼脂糖凝胶电泳检测，结果显示：各组样品总DNA25kb左右，每组样品条带明亮、清晰、整齐、无降解现象，提取效果较好；而阴性对照组未出现条带。

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The oxidative modification of LDL was identified by agarose gel relative electrophoretic mobility ( REM) and fluorescence of thiobarbituric acid reaction substances ( TBARS) contents.

测定LDL的电泳迁移率及硫代巴比妥酸反应物质含量。

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Research on preparation of spherical agarose gel and coupling reaction with diethylaminoethyl

琼脂糖凝胶的制备及化学改性研究

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Results The recombinant plasmid cut by incision enzyme EcoR I and Kpn I overnight generated a 0.78 kb fragment and a 4.68 kb fragment in 1% agarose gel electrophoretogram.

结果重组质粒通过双酶切产生了0.78kb目的插入片段及4.68kb载体片段；

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From the agarose gel electrophoresis of the genomic DNA, which extracted by different concentrations of ammonium acetate solution, we could see that there were no significant differences between the amounts of the DNA.

不同浓度醋酸铵溶液沉淀所得基因组DNA的琼脂糖凝胶电泳结果显示三种浓度醋酸铵溶液提取的DNA量无显著性差异。

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It was a way of antenatal diagnosis and gene diagnosis for patients with or without symptom of spinocerebellar ataxias. Results: The gene types of spinocerebellar ataxias could be a preliminary determined by 3% agarose gel electrophoresis.

结果：1.所采用的PCR扩增方法能够进行脊髓小脑性共济失调1-3,6-7和12的基因诊断。3%琼脂糖凝胶电泳可用于初步判定患者的基因亚型。

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Agarose gel electrophoresis was used to detect DNA fragmentation. Western blots were used to measure protein expression level of several genes related to apoptosis.

琼脂糖凝胶电泳检测DNA梯带：免疫印迹法检测凋亡相关基因蛋白的表达水平。

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The PCR products were further analyzed by agarose gel electrophoresis and single strand conformation polymorphism ( SSCP) analysis. The PCR products showing polymorphism by SSCP analysis were directly sequenced. Suspectable exons were analysed with inversion sequence.

以琼脂糖凝胶电泳鉴定产物，作单链构象多态性（SSCP）分析检测PCR产物，对迁移率异常者进行DNA直接测序。

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the results of 1% agarose gel electrophoresis and ultraviolet spectrophotometer analysis show that the total rna of bauhinia variegata extracted through this method has no obvious degradation and has a good purity.

所得RNA样品经紫外分光光度计检测和1%琼脂糖凝胶电泳分析证明无明显降解，且具有较高的纯度。

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Method 14 samples of the supernatants of throat swab samples and the cultural supernatants of throat swab from infected rats are performed PCR analysis using animal mycoplasms routine primers and M. Pulmonis species-specific primers respectively. The PCR products are confirmed with 2% agarose gel electrophoretic analysis.

方法使用支原体通用引物及鼠肺支原体特异性引物对14份大鼠喉气管拭子洗液和拭子支原体培养液进行PCR扩增，2%琼脂糖电泳鉴定。

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Agarose gel electrophoresis of DNA samples from K562 cells exposed to Artesunate with a concentration from 4 to Sug/ ml showed obvious ladder strips.

在4一sug/m1浓度组的K562细胞DNA凝胶电泳可见明显的梯形条带。

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DNA was shown to form stable and positively charged complexes with cationic nanoparticles at N/ P ratios above 2 by agarose gel electrophoresis, fluorescence displacement, particle size and zeta potential measurements.

琼脂糖凝胶电泳、荧光置换、粒径和电位测试的结果表明在N/P比2以上时，DNA与阳离子纳米粒形成了稳定且带正电的复合物。

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With the specific primers and high-fidelity DNA polymerase applied, PCR product was detected by agarose gel electrophoresis. 2.

应用设计的特异引物，利用高保真的DNA聚合酶进行扩增，PCR产物利用琼脂糖凝胶电泳鉴定。

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Apoptosis of cancer cells was evaluated by terminal uridine deoxynucleotidyl nick end labeling ( TUNEL) and agarose gel electrophoresis, respectively.

并于转染不同时间后收集细胞，分别采用琼脂糖凝胶电泳和TUNEL方法检测肝癌细胞的凋亡情况。

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PCR amplification of the target gene and PCR reaction products obtained by agarose gel electrophoresis detection, and for the conduct of amino acid sequence and bioinformatics analysis.

目的基因的PCR扩增，并将所得PCR反应产物通过琼脂糖凝胶电泳检测，用以进行氨基酸序列和及其生物信息学的分析。

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At the same time, separation and purification of puerarin using β-cyclodextrin-coupled agarose gel media is becoming the key development and direction for its advantage with green environment protection and low cost.

同时，指出β-环糊精键合固定相技术因具有绿色环保和低成本的优点，而成为发展的重点和方向。

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Methods: YAP element was detected by PCR amplification and agarose gel electrophoresis.

方法利用PCR法对此位点进行扩增,经过琼脂糖凝胶电泳检测结果.

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