OEG

Objectives To observe olfactory ensheathing glia ( OEG) survival and repair in vivo for spinal cord injury after OEG transplantation.

目的研究嗅鞘细胞（OEG）移植后在体内存活情况以及对大鼠脊髓损伤长期的修复作用。

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For spinal cord contusion and transection, OEG can promote the axons regeneration and the recovery of locomotion function in our study.

OEG移植具有明显促进脊髓挫伤加横断伤神经纤维再生和功能恢复的作用。

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Methods The OEG was cultured with the olfactory bulb of Wistar neonate rats.

方法用新生Wistar大鼠嗅球做OEG培养，大量增殖并标记。

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Cell culture: The primary OEG was cultivated with the olfactory bulb of Wistar neonate rats, and then the purification was done by free serum medium.

细胞培养：新生Wistar大鼠嗅球（OB）做OEG原代培养，然后利用无血清培养基添加一些促进生长的微量物质做纯化培养，为OEG提供了合适的生长环境而大量增殖。

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OEG proliferated quickly by free serum medium, and cell count increased by 16 times of primary culture through 9 days cultivation. Its purity is 85%.

通过无血清纯化培养，OEG增殖迅速，原代培养9天，细胞数目可达16倍以上，纯度可达85%。

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She also said Kroeger was happy to let his bride-to-be take charge.

她还说Kroeger很高兴让他的准新娘做主。

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