wnv

Results 0.02 PFU/ ml and 2 PFU/ ml of WNV RNA were detected by real-time and conventional RT-PCR assay, respectively. Sensitivity of the former was 100-fold greater than those of the latter.

结果采用所建立的实时RT PCR法和常规RT PCR法可分别检测到0.02PFU/ml和2PFU/ml的西尼罗病毒RNA，前者的敏感性比后者高100倍。

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West Nile fever and encephalitis caused by West Nile virus ( WNV) is maintained in a natural transmission cycle involving mosquito vectors and bird reservoir hosts, with the fatality of 4%~ 11%.

西尼罗病毒（West nile virus，WNV）感染是一种经蚊虫传播、以鸟类为主要动物宿主的自然疫源性疾病，在临床上可表现为西尼罗热或西尼罗脑炎，病死率4～11%。

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Can inhibit replication of DENV and WNV, RIG-I was transiently over-expressed in A549 cells followed by infection with DENV or WNV, and the progeny virus yield decreased sharply in a dose-dependent manner.

我们进一步通过过表达的方法观察了RIG-I对登革病毒和西尼罗病毒复制的抑制效果，结果发现RIG-I在A549细胞中的过表达能够显著降低子代病毒的产量，并呈剂量依赖性。

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This article reviews the current researches in WNV regarding its infection, immunity and epidemiology.

本文就近几年来对西尼罗病毒的感染、免疫与流行病学方面的研究进展进行了综述。

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In Europe, Africa, Middle East and Asia, mortality in birds associated with WNV infection is rare.

在欧洲、非洲、中东和亚洲，西尼罗河病毒感染引起的鸟类死亡很罕见。

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There is one reported case of transplacental ( mother-to-child) WNV transmission.

曾有一例西尼罗河病毒经胎盘（从母亲到婴儿）传播的报告。

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IgM can be detected in nearly all cerebrospinal fluid ( CSF) and serum specimens received from WNV infected patients at the time of their clinical presentation.

当患者感染西尼罗河病毒并出现临床表现时，几乎可以在所有收集的脑脊液（CSF）和血清样本中发现IgM。

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Mosquitoes were proved as primary vectors involved in the transmission cycle of WNV.

研究证实蚊虫为其主要的传播媒介.

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Transfusion-transmitted West Nilevirus ( WNV) infections were first reported in2002.

2002年，首次报道输血传播西尼罗河病毒（WNV）。

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Using the recombinant envelope protein of WNV as antigen, the serum antibody was detected by ELISA.

方法利用构建的包膜蛋白重组质粒（pQE-30）表达纯化西尼罗病毒包膜蛋白，以此为抗原进行ELISA检测。

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Generally, WNV is spread by the bite of an infected mosquito.

一般而言，西尼罗河病毒是由受到感染的蚊子传播的。

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West Nile virus ( WNV) within the Flavivirus genus of the family Flaviviridae, cause human severe diseases ranging from West Nile fever to West Nile encephalitis or meningitis.

西尼罗病毒（West nile virus）属于黄病毒科黄病毒属，该病毒感染人类可导致西尼罗热、西尼罗脑炎、脑膜炎等严重疾病。

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About one in150 people infected with WNV will develop severe illness.

而大约每150名感染者中会有1人发展成严重疾病。

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WNV is maintained in mosquito populations through vertical transmission ( adults to eggs).

西尼罗河病毒通过纵向传播（成虫到卵）在蚊子群体中生存。

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All results demonstrated that the DNA-based WNV replicons could express exogenous genes and replicate itself well. This may set a foundation for following observation of replicon immunogenicity. 2.

这些结果说明，DNA形式的西尼罗病毒复制子能够表达外源基因，具有良好的复制活性，为下一步观察该复制子的免疫原性奠定了基础。

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There is no specific treatment for WNV infection.

目前对西尼罗河病毒感染还没有特定的治疗方法。

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Objective To investigate the biological characteristics of an imported West Nile virus ( WNV) strain, covering morphology, pathogenicity, cell line sensitivity.

目的对引进的西尼罗病毒（WNV）毒株的形态学、致病性、细胞敏感性、免疫原性等生物学性状进行探讨，为进一步开展WNV的相关研究奠定基础。

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Transmission of WNV to laboratory workers has been reported.

曾有西尼罗河病毒传给实验室工作人员的报告。

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Objective To investigate the community structure and dynamic of potential vector of West Nile Virus ( WNV) Disease in Jiangsu Province.

目的了解江苏省西尼罗病毒病潜在蚊媒群落构成及季节消长情况，为及时有效地切断西尼罗病毒病传播途径提供科学依据。

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