pfx

Two pair of primers containing attB adapter were designed separately by Gateway cloning technology. Platinum pfx DNA polymerase which can reduce the non-specific binding greatly was used during two PCR in which adding B sequence to the cloned gene.

根据Gateway克隆技术的要求，设计含有attB接头的引物，利用高保真的PlatinumpfxDNA聚合酶，通过PCR方法在克隆基因的两端加上B序列。

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These results suggested that the bactericidal actions of NFX, ENX, and PFX are all interrelated with their inhibitory effects on bacterial DNA synthesis, and continuous RNA and protein synthesis are prequisites for the bactericidal action.

以上结果提示：氟喹诺酮药物的杀菌作用与其抑制细菌DNA合成有关，且须以RNA和蛋白质不断合成为前提条件；

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The normal spectrum value of gurgling sound in 30 healthy equus in Tianjin area detected using microcomputer ( CYPFX software system).

应用微机CYPFX软件系统，对天津地区30匹健康马骡肠鸣音频谱正常值作了测定。

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Derived certificate is one is suffixal file with PFX.

导出的证书是一个以PFX为后缀的文件。

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Enter the name of a PFX file.

输入pfx文件的名称。

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