1、

Taq DNA polymerase is an ideal enzyme for DNA sequencing because it's fast, highly processive and active over a broad range of temperature.

taq DNA聚合酶具有反应速度快、温度作用范围广及良好的续进性等特点,可视为一种理想的DNA顺序分析酶。

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2、

Study on the Functional Domain of Taq DNA Polymerase

taq DNA聚合酶功能区域的定位

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3、

High content of protein, phenol, tannin, pigment and polysaccharide in plant tissues usually affect the activity of Taq DNA polymerase and Lead to failure of PCR reaction.

植物体内所含的蛋白质、单宁、酚类、多糖及色素等次生代谢物质影响taq DNA聚合酶的活性,是导致PCR扩增反应失败的主要因素。

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4、

In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~ ( 2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction.

为建立适宜的SSR反应体系,对影响SSR-PCR扩增的模板DNA浓度、Mg~(2+)的用量、taq DNA聚合酶浓度、dNTPs浓度、退火温度等因素进行了优化。

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5、

After Taq DNA polymerase had been isolated and purified from the thermophilic bacteria ( Thermus aquatics) in aquatic habitat by Randall K. Saiki and his colleagues in 1988, it was widely used in PCR techniques.

1988年,Randall K.Saiki等[1.2]从水生栖热菌(Thermus aquatics)中分离纯化到了taq DNA聚合酶,之后它广泛应用于PCR技术。

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6、

Moreover, the concentrations of Mg2+, dNTP and Taq DNA polymerase in allele-specific PCR were higher than that in conventional PCR.

在等位基因特异PCR中,Mg2+、dNTP及taq DNA聚合酶的用量均大于普通PCR。

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7、

Rapid Purification of High-activity Recombinant Taq DNA Polymerase Using Freezing-thawing Method

以冻融法快速纯化高活力基因工程taq DNA聚合酶

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8、

One of the mechanisms causing gene mutation might be to change of the Taq DNA polymerase conformation. It interfers with the enzyme-substrate-dNTP binding site and further causes replication mismatch.

这一实验结果也说明了稀土是一种诱变剂,引起基因突变的机制之一可能是改变taq DNA聚合酶的立体构象,干扰了酶与底物dNTP结合位点,从而引起复制错配。

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