1、

Method: Through indirect immunofluorescence technique, 13 kinds of cytokeratin antibodies and the vimentin monoclonal antibody were employed as a negative control antibody in this study.

方法:应用间接免疫组化技术,将13种细胞角蛋白抗体和阴性对照剂波形蛋白单克隆抗体与REEC-1细胞反应。

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2、

After S10 was expressed, the products were detected and analyzed by the SDS-PAGE, Western-blot and Immunofluorescence technique.

多角体蛋白基因表达后,用SDS-PAGE、Western-blot和免疫荧光技术对表达产物进行了检测。结果表明:S10原核表达质粒、重组杆状病毒成功获得;

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3、

Methods: The technique of serum free cultivation and the methods of immunofluorescence cytochemistry were used.

方法利用无血清培养技术和细胞免疫化学方法.

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4、

Study on bridged avidin biotin technique improving the sensitivity of phycobiliprotein immunofluorescence assay

桥式生物素-亲合素标记提高藻胆蛋白免疫荧光法灵敏度的研究

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5、

Direct immunofluorescence staining technique using Gardnerella monoclonal antibody and Gram stain were used to examine the smears of abnormal vaginal secretion of 247 outpatients in Gynecology Department. Gardnerella vaginitis ( GV) was found in 6 cases ( 2.4%).

应用单克隆抗体直接免疫荧光染色,在妇科门诊247例异常阴道分泌物涂片中检出加德纳菌感染者6例(2.4%),诊断为加德纳杆菌性阴道炎。

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6、

[ Methods] 15 monoclonal antibodies ( McAbs) against T s muscle larva ( ML) soluble antigens were obtained by using hybridoma technique. The reactivity of monoclonal and polyclonal antibodies were tested by ELISA, Western blotting and indirect immunofluorescence assay ( IFA).

[方法]采用杂交瘤技术,获得15株特异性单克隆抗体,随后用酶联免疫吸附试验(ELISA)、免疫印迹法(WESTERN BLOTTING)和间接免疫荧光试验(IFA)对部分免疫显性抗原进行分析。

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7、

Anti-neutrophil cytoplasmic antibody ( ANCA) was detected with indirect immunofluorescence, and the pathological changes were investigated with pathological tissue section technique.

用间接免疫荧光法检测抗中性粒细胞胞浆抗体(ANCA),病理组织切片技术观察病变。

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