1、

Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction ( PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.

阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。

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DNA Sequencing of Variable Regions of Monoclonal Antibodies

单克隆抗体可变区DNA序列的测定

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3、

Methods Antibiotic susceptibility was tested by the disk diffusion to screen out the strains that produced the ESBLs; conjugative transfer test, plasmid profile, PCR and DNA sequencing were used to detect the phenotype or genotype of STEC.

方法采用纸片琼脂扩散法筛选36株产志贺毒素大肠埃希菌产ESBLs菌株,接合传递实验、质粒谱分析、PCR检测耐药基因和DNA序列分析产ESBLs菌株的表型和基因型。

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4、

But advances such as gene editing and next-generation DNA sequencing allow scientists to weaponise new viruses, perhaps including custom pathogens engineered to overcome existing immunities or to be impervious to current drugs.

但基因编辑和新一代DNA测序等技术进步使得科学家可以把新病毒变成武器,或许包括用定制病原体攻破现有免疫系统或抵抗现有药物的影响。

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5、

The problem addressed in this paper is concerned with DNA sequencing by hybridization ( i.e. SBH), in which the estimate error of target DNA length and errors of hybridization are allowed.

本文讨论了允许长度估计误差和杂交错误的更实际SBH(Sequencing by Hybridization)最优重构问题。

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6、

With the development of DNA sequencing, molecular systematics have infiltrate through more and more research field of bryophyte in recent years.

伴随着DNA测序技术的发展而兴起的分子系统学研究手段近年来越来越多的渗透到苔藓植物学各研究领域。

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8、

DNA sequencing technology is one of the core technologies of life science research.

DNA序列测定技术是现代生命科学研究的核心技术之一。

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9、

Reverse transcription-polymerase chain reaction was employed to evaluate the expression of PRL mRNA in pituitary glands, and DNA sequencing was used to analyze the mutation in regulatory sequence of PRL gene.

应用RT-PCR方法检测PRL基因的表达,同时克隆PRL基因5'上游调控序列-1206~-1966bp片段,采用DNA直接测序方法检测该序列的突变情况。

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10、

In order to determine the characteristics of the highly repeated sequence of insectivora, a highly repeated DNA fragment of hedgehog ( one Insectivora animal) has been studied by molecular cloning and DNA sequencing. 1186 bp sequence has been obtained.

为了分析比较食虫目高重复顺序的结构特征,我们将食虫目动物刺猬的高重复顺序DNA进行了分离、纯化、分子克隆,并测出了1186bp的核苷酸顺序。

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Results Restriction enzyme digestion and DNA sequencing suggested that the recombinant expression plasmid contained correct encoding region of Pokemon, without reading frame shift.

结果限制性核酸酶切及序列分析表明重组表达质粒包含Pokemon基因编码区,阅读框架无移位;

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This is the first report of vertical transmission of CT proved by DNA sequencing.

提示:首次从DNA测序分子水平证实母婴垂直传播CT,垂直传播CT在重庆地区颇为常见。

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13、

methods the mutation of mitochondrial dna from all 18 family members of a chinese pedigree with maternally inherited aminoglycoside antibiotic-induced deafness was detected by pcr and dna sequencing.

方法应用PCR和DNA序列分析技术,对一个有明确氨基糖苷类抗生素应用史的母系遗传耳聋家系共18人(包括聋人和听力正常者)的线粒体DNA进行研究。

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Researchers at Johns Hopkins University in the U.S.used the cutting-edge DNA sequencing and identified the radical genomic rearrangements that occur only in tumors, not in healthy cells.

美国约翰斯·霍普金斯大学的研究人员们使用前沿的DNA测序,辨识只发生在肿瘤中而不是在健康细胞中的自由基基因组重排。

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DNA sequencing results further confirmed that RT-PCR products were truly MAGE cDNA.

DNA测序结果表明RT-PCR产物为MAGE-1、-3和-4基因。

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There was abnormal gene product in 1 AMOL cell line out of 12 AML cell lines with Flt-3 positive expression ( positive rate 8.3%). DNA sequencing of abnormal gene product showed two coding duplication sequence with 29 bp long.

12例Flt-3表达阳性的AML中有1例AMOL细胞株存在有异常表达(阳性率为8.3%),测序显示存在有29bp的两个编码重复序列,其余Flt-3表达阳性的细胞株未检出重复序列。

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17、

The results from heteroduplex mobility assay ( HMA) were compared with HIV 1 subtype results determined by DNA sequencing.

并将这种异源双链泳动分析法(heteroduplexmobilityasay,HMA)的结果与DNA序列测定结果比较。

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18、

DNA sequencing found that the aberrant transcripts of FHIT gene were caused by deletion or insertion of nucleotide fragments.

DNA测序发现FHIT基因的异常转录本是由于核普酸片段的缺失和插入突变引起的;

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19、

DNA sequencing results showed that bkt was composed of 960 bp and encoded 320 amino acid residues.

序列测定结果表明,bkt全长960bp编码320个氨基酸。

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20、

With PCR-mapping, DNA sequencing and bioinformatics approach, structure of an integron in a clinical isolate of Pseudomonas aeruginosa resisting to imipenem was studied.

运用PCR mapping、DNA测序技术和生物信息学手段,对一株临床分离耐亚胺培南铜绿假单胞菌进行整合子结构分析。

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