1、

Methods The endometrial stromal cells were cultured in DMEM/ F12 medium containing different concentrations of IL-6 or IL-8 for 24 hours, then the media were collected to measure the levels of MMP-9 by ELISA.

方法分别用含不同浓度的IL-6和IL-8以及无干扰因素的培养液培养各组人子宫内膜基质细胞24h后,ELISA方法测定培养液中MMP-9的含量。

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2、

The cells in the control group were added with DMEM/ F12 nutrient fluid of EGF, bFGF and B27 to culture in 5% CO2 incubator for one week at 37 ℃.

对照组细胞中直接加含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM/F12培养液,37℃,体积分数为0.05的CO2培养箱培养1周。

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3、

Control group I ( SC medium+ NSC+ DMEM/ F12);

试验对照组1(SC培养基+NSC+DMEM/F12);

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5、

The length of process in the fetal calf serum+ DMEM/ F12 group was longer significantly than that in the newly calf born serum+ fetal calf serum+ DMEM/ F12 group in 4 days ( P < 0.01);

在4d内胎牛血清+DMEM/F12组细胞突起长度明显优于新生牛血清+胎牛血清+DMEM/F12组(P<0.01);

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6、

Methods PC12 cell was irradiated with DMEM/ F12 agent of enriched uranium, and the internal exposure doses were calculated.

方法在PC12细胞中加入浓缩铀DMEM/F12工作液,计算PC12细胞的内照射吸收剂量。

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7、

Methods NSCs harvested from the cerebral cortex of neonatal one-week mice were triturated and cultivated in serum-free DMEM/ F12+ bFGF+ EGF+ B27, then identified by the immunohistochemistry SABC method.

方法取新生1周内乳鼠的大脑皮质,机械分离出神经干细胞,无血清培养液(DMEM/F12+bFGF+EGF+B27)培养,免疫组化SABC法对神经干细胞进行鉴定;

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8、

Samples in the normal control group and the model control group were cultured in serum DMEM/ F12 ( containing 0.2 fetal calf serum) without growth factor for 7 days to induce the differentiation.

正常对照组和模型对照组在加入去除生长因子的血清DMEM/F12(含体积分数为0.2的胎牛血清)中培养7d进行诱导分化。

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9、

The culture results of the porcine ear skin fibroblasts was good by using DMEM/ F12 containing 20% fetal bovine serum.

在培养条件方面选取DMEM/F12培养液+20%胎牛血清培养猪耳皮肤成纤维细胞,效果较好。

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10、

Umbilical artery rings and umbilical vein rings embedded in three-dimensional collagen gels in DMEM/ F12 ( 1:1) serum-free medium grew well. The new outgrows were positive by CD34 immunohistochemistry.

脐动脉环和脐静脉环在胶原凝胶和DMEM/F12(1:1)无血清培养基中生长良好,CD34免疫组织化学鉴定其为新生的血管。

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11、

At the third-generation ( F3) cells continued to be cultured ( 10% FBS, DMEM/ F12), and the cell population is divided into intervention group and control group, the experimental group were added to different concentrations of bFGF.

取第3代(F3)细胞继续进行培养(10%FBS,DMEM/F12),并将该细胞群分为干预组和空白对照组,实验组分别加入不同浓度梯度的bFGF。

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12、

After digestion, cells were cultured in DMEM/ F12 supplemented with 20% fetal calf serum.

将消化后得到的细胞用含20%胎牛血清的DMEM/F12培养基进行培养。

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13、

Method To take sciatic nerves in 3-4 days SD rats and purify Schwann cells. A group: DMEM/ F12 contained 10% calf blood serum. B group: conditioned medium of macrophages activated by self-neural homogenate.

方法取3-4dSD乳鼠坐骨神经,纯化培养许旺细胞,A组加入含10%胎牛血清的DMEM/F12培养基,B组加入自体神经匀浆激活的巨噬细胞条件培养基;

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15、

Control group III ( NSC+ DMEM/ F12).

试验对照组3(NSC+DMEM/F12)。

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16、

Group B: fibroblasts+ Schwann cells+ DMEM/ F12;

B组:成纤维细胞+雪旺细胞+DMEM/F12;

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17、

Basal medium was composed of penicillinum, phytomycin, amphotericin B and DMEM/ F12 containing B27 annex solution of 0.02 volume fraction.

基础培养基组成:含青霉素、链霉素、两性霉素B及体积分数为0.02B27添加液的DMEM/F12。

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18、

Methods Nucleus pulposus tissue taken from a one-month-old rabbit was treated by Trypsin and collagenase, and then the cells were cultured in DMEM/ F12 medium.

方法取一月龄新西兰兔的髓核,胰酶和胶原酶消化,DMEM/F12培养基中培养,倒置显微镜观察细胞形态。

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19、

Conclusion: Human umbilical vessel ring culture model of three dimensions in DMEM/ F12 ( 1:1) serum-free medium has been established.

结论:用无血清培养基和Ⅰ型胶原可以立体培养脐血管环。

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20、

The collected fat mesenchymal stem cells and cell nucleus pulposus with DMEM/ F12 ( 1: 1) medium monolayer culture respectively. 2.

所收集的脂肪间充质干细胞和髓核细胞用DMEM/F12(1:1)培养液分别单层培养。

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