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Objective Aim to set up the method of glycoprotein B genotype of human cytomegalovirus ( HCMV) analysis by using nested polymerase chain reaction ( nPCR) and restriction fragment length polymorphism ( RFLP).

目的建立人巨细胞病毒(HCMV)糖蛋白B(gB)基因的套式聚合酶链反应(nPCR)加限制性长度多态性分析(RFLP)的分型方法。

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RFLP ( restriction fragment length polymorphism) analysis showed that the transposon is 3 cM ( centiMorgan) away from the S locus and retains high transpositional activity with a germinal excision frequency of 20%.

限制性酶切片段长度多态性(RFLP)的分析证明该转座子距S位点约3cM(centiMorgan),并且保持了较高的转位活性,其配子切除频率高达20%。

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Bacterial community and diversity in water sample from a oil reservoir was characterized by restriction fragment length polymorphism ( RFLP) of 16S rRNA genes.

通过16S RRNA基因的限制性酶切片段长度多态性分析(RFLP)方法,考察了油藏水样中细菌群落及多样性。

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Methods HPV DNA was detected using polymerase chain reaction ( PCR) with a consensus primer and typed by using restriction fragment length polymorphism ( RFLP) method in 151 paraffin-embedded biopsy specimens from seven kinds of external genital epidermal proliferative lesions.

方法采用通用型引物PCR方法对151例外生殖器表皮增殖性病变进行HPV DNA的检测,应用限制性内切酶片段长度多态性(RFLP)方法进行HPV DNA分型。

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The mutation was confirmed by restriction fragment length polymorphism ( RFLP).

针对所发现的突变,以限制性内切酶片段长度多态性(RFLP)分析加以验证。

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Three random clones from the genomic library and one rDNA clone of Armillaria mellea were used to probe for restriction fragment length polymorphisms ( RFLP).

用文库中的三个随机克隆以及蜜环菌(Armillaria mellea)的一个rDNA克隆作探计,检测香菇的RFLP。

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