1、

The recombinant DHFR protein containing his-tag was purified by using TALON metal affinity resin under nondenaturing conditions.

在非变性条件下,用TALON金属亲和层析树脂纯化含组氨酸标记的重组DHFR。

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2、

The urea-induced equilibrium unfolding transition of dihydrofolate reductase ( DHFR) from chicken liver was well described by a two-state unfolding model involving only native and unfolded forms.

鸡肝二氢叶酸还原酶(DHFR)平衡态的去折叠曲线符合二态模型,但在4.0mol/L脲中的去折叠动力学为两相。

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3、

Construction of GPI-anchored Superantigen SEA and Expression in CHO-dhfr-Cells Membrane

超抗原SEA的GPI锚定修饰和在CHO-dhfr~-细胞膜上的表达

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4、

Analysis of the DHFR and DHPS Genes of Pneumocystis Carinii Infected SD Rats

大鼠卡氏肺孢子虫DHFR和DHPS基因序列的测定与分析

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5、

the level of dihydrofolate reductase ( dhfr) mrna expression was assayed with rt pcr.

用RT-PCR检测二氢叶酸还原酶(DHFR)mRNA表达量;

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6、

Analysis and expression in CHO/ dhfr~+ of recombinant human protein C

重组人蛋白C在CHO/dhfr~+细胞中的表达与分析

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7、

Stable eukaryotic expression of rhIL 12 in CHO dhfr-cells

重组人细胞介素12基因在CHO&dhfr~-细胞的稳定表达

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8、

Construction of targeted integration and high expression system in CHO/ dhfr-cell line

新型CHO/dhfr~-细胞定点整合和表达系统的建立

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9、

After PCR and sequencing, the expression plasmid pWD κ H was transfected into CHO ( dhfr ~-) cells.

经PCR和测序鉴定正确后, 用lipofectAMINE2 000转染CHO ( dhfr - ) 细胞.

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10、

The products of TS gene and DHFR gene were 403 and 273 bp respectively.

结果卡氏肺孢子虫TS基因和DHFR基因的扩增产物分别为403和273bp。

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