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The Preparation of Monoclonal Antibodies Against CSFV-C Strain and Development of Rapid Test Strip for CSFV

猪瘟C株单克隆抗体制备和猪瘟胶体金快速诊断试纸条的研制

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Our research provided a basis to develop sub-unit vaccine and diagnostic antigen against CSFV.

该研究为研制防治猪瘟的亚单位疫苗与诊断试剂盒奠定基础。

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Based on published sequences of strain C of CSFV, a specific PCR primer pair P1 N1 and a half nested PCR reverse primer N1 ′ were designed.

据已发表的CSFV C-株序列,设计合成了5′-UTR特异性PCR引物对P1-N1及半套式下游引物N1′。

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Based on the published sequences of CSFV, 26 nested and half nested PCR primers covering the genome sequence of C strain were designed.

根据已发表的猪瘟病毒(CSFV)序列设计并合成了26条覆盖全长基因组的套式和半套式PCR引物。

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potentiating effect of immunologic activity factor on inoculating by csfv vaccine

免疫活性因子增强猪瘟疫苗免疫效果的试验研究

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the study will lay a foundation for csfv genetic engineering vaccine.

本试验为猪瘟基因工程疫苗的研制奠定基础。

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One set of specific primers were designed according to the conserved sequence of E2 gene of CSFV in GenBank and a standard RT-PCR was founded to detect the infection of CSFV. Compared with rabbit-cross test, 7 samples from pigs suspected infection with CSFV were detected with RT-PCR.

通过计算机软件设计了猪瘟病毒E2基因保守区的1对引物,建立了猪瘟病毒RT PCR检测方法,同时应用兔体交叉试验进行对比,对7份临床送检病例进行了检测。

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High Expression of CSFV E0 Protein and Establishment of an Indirect ELISA for Detection of Antibodies

猪瘟病毒E0蛋白的高效表达及其间接ELISA检测方法的建立

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Detection of CSFV C strain by immunogold silver staining technique

应用免疫金银技术检测猪瘟病毒兔化弱毒株感染细胞

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The results indicated that infectious RNA of CSFV could be obtained from constructed full-length cDNA.

结果表明,所构建的全长cDNA具有感染性。

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Research and Development Trend for the Detection of CSFV Antibody

猪瘟抗体检测技术的研究现状及发展趋势

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Retroviral Vector Mediated CSFV E2 Envelope Protein Gene Expression in Eukaryotic Cells and Animal Immune Experiment

逆转录病毒载体介导的猪瘟病毒E2基因在真核细胞中表达及动物免疫试验初报

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Preliminary study on multiplication characteristics of epidemic CSFV of Guangxi province

广西猪瘟流行毒株生长特性的初步研究

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Construction of eukaryotic expression vector of CSFV E2 genes and its secretory expression

猪瘟病毒囊膜蛋白E2基因真核分泌型表达载体的构建及其表达

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Analysis of the genome structure of CSFV by bioinformatics

用生物信息学手段辅助分析猪瘟病毒的基因结构

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Construction and Expression of the E2 of CSFV and Swine IgG Heavy Chain Fused Gene

猪瘟病毒保护抗原E2和猪IgG重链基因的合成及其表达蛋白

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Cloning of E_2 gene of CSFV and construction of Pichia pastoris transfer vector

猪瘟病毒分离株E2基因的克隆与酵母表达系统转移载体的构建

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Expression of the Main Antigenic Domains of CSFV E2 Protein in E.coli and Primary Application

猪瘟病毒E2蛋白主要免疫显性抗原多肽原核表达及初步应用

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The results showed that the assay has high sensitivity to CSFV positive serum comparing with negative serum.

实验证明,该方法对标准的CSF阳性及阴性血清的具有良好的敏感性。

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