ligase

study on diagnosis of neonatal chlamydia trachomatis pneumonia by ligase chain reaction enzyme linked immunoadsorbent assay

小婴儿沙眼衣原体肺炎的连接酶链反应&酶联免疫吸附法诊断研究

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Effect of Arsenic on Human DNA Ligase

砷对人DNA连接酶影响的研究

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Two genes encoding a NAD+-dependent DNA ligase ( LigA) and an ATP-dependent DNA ligase ( LigB) were identified in the genome of the extremely radioresistant bacterium. Deinococcus radiodurans ( DR).

在耐辐射球菌基因组中有两个基因分别编码DNA连接酶，一个是NAD~+依赖型DNA连接酶（LigA），一个是ATP依赖型DNA连接酶（LigB）。

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In this study, HCV positive serum samples, cell-cultured avian influenza virus subtype H5N1 and SARS virus samples were used to evaluate the detection ability of rolling circle amplification with circle probes, which can hybridize with target molecules and be circularized by T_4 DNA ligase.

本文中用可特异性连接环化的寡核苷酸链作为探针，分别进行了1份细胞培养的禽流感病毒H5N1亚型样品、1份细胞培养的SARS病毒样品和4份丙型肝炎病毒阳性血清样品的检测。

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A highly sensitive and simple fluorimetric method for the determination of ATP based on ATP-dependent T4 DNA ligase has been developed.

利用T4DNA连接酶对ATP的高度依赖性，我们发展了一种ATP检测新方法。

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Finally, DNA ligase newly synthesized DNA chain and the original links.

最后DNA连接酶将新合成的DNA链与原来的链接上.

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Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.

方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。

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Objective: To develop a new nucleic amplication method for detection of Chlamydia trachomatis DNA by gap ligase chain reaction ( G-LCR).

目的：建立缺口连接酶链反应方法检测沙眼衣原体。

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Real-time Visualization of Ligation Reaction Catalyzed by E.coli DNA Ligase Based on Molecular Beacon

基于分子信标实时监测大肠杆菌DNA连接酶催化的DNA连接过程

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Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.

方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.

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All synthetic fragments were annealed and ligated with T4 DNA ligase to form double DNA chain. This synthetic gene was recombined with P-Blue script as vector and transformed into E.

分离纯化后的片段连接成完整的双链基因，与P-Blue script重组，转化E。

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fancl was then found to be an ubiquitin e3 ligase, which is involved in dna damage repair [ 4-6].

FANCL是一种含有PHD结构域的泛素E3连接酶，参与DNA的损伤修复。

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A simple, rapid and reproducible method has been developed for the production of highly purified T4 DNA ligase in good yield from induced lysogens of λ T4 lig phages. Heparin-sepharose 4B and blue dextran-sepharose 4B affinity chromatographic columns were used to purify the enzyme.

我们采用简便、快速、重复性好的肝素-琼脂糖和蓝色葡聚糖-琼脂糖柱两次亲和层析方法，纯化得到了高纯度的T4-DNA连接酶。

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Thus, the NEU-induced primary chromosome breakage may not be repaired by the ligase.

由NEU所诱发的染色体初级断裂不能为连接酶所修复。

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But the electroporation efficiency of plasmid in ligation system is relatively low mainly caused by T4 DNA ligase.

连接体系中质粒的转化效率较纯质粒低，而T4DNA连接酶是影响其转化效率的主要因素。

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The effect of arsenic on human DNA Ligase one of key enzyme of DNA repair, was studied by using purified enzymes and human cells in culture.

采用纯化酶活性分析和人细胞体外培养方法，研究砷对人DNA修复关键酶&DNA连接酶活性的影响。

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The linear plasmid and the designed fragment ( afp) were directively ligated with T4 DNA ligase, and the plant expression vector of carrot aTh was constructed.

将目的片段与线性质粒在T4DNA连接酶的作用下进行定向连接，构建成胡萝卜afp的植物表达载体pBAF。

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Recent Advances in DNA Ligase Isozymes

DNA连接酶同工酶的研究进展

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