a flame-retardant nitrogen-containing curing agent was synthesized by melamine, formaldehyde and phenol through two reactions.

1、

then in the same way, we prepared the sulfonic mesoporous molecular sieve in two steps.

随后用同样方式，通过两步反应，合成磺酸型MCM-41介孔分子筛。

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2、

hiv-1 integrase ( in) integrates viral dna into host cells through two steps metal ions-dependent reactions.

HIV-1整合酶（IN）通过依赖金属离子的两步反应将病毒DNA整合入宿主细胞中。

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3、

methods the target compound was synthesized by two steps with 4-hydroxy-6-methyl-2-pyrone and n, n-dimethylformamide dimethyl acetal as the starting materials.

方法以4羟基6甲基2吡喃酮、N，N二甲基甲酰胺二甲氧基缩醛为起始原料，经两步反应制得目标化合物。

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4、

study on ch_4-co_2 two-step reaction and co_2 hydrogenation by a short contact way

短接触CH4-CO2两步反应和CO2加氢研究

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5、

diagnostic significance of plasma prolidase activity in chronic liver diseases

血浆脯氨酸肽酶活性测定在慢性肝病诊断中的意义

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6、

a modified method for superoxide dismutase activity measurement. dai

超氧化物歧化酶活性测定的一种改进法

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7、

research of serum γ-glutamyltranspeptidase in laryngeal cancer diagnosis and prognosis

喉癌患者血清γ－谷氨酰转肽酶活性测定

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8、

detection and analysis of serum prolidase activity in viral hepatitis

病毒性肝炎患者血清脯氨酸肽酶活性测定及其意义分析

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9、

studies on testing conditions of-d-glucosidase activity in tea

茶叶中β-D-葡萄糖苷酶活性测定条件的研究

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10、

measurement of serum biotinidase activity in hepatic disease and its significance

肝硬化患者血清生物素基酶活性测定及其临床意义

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11、

research on chromogenic peptide in determination of prekallikrein/ kallikrein activity

发色多肽用于前激肽释放酶活性测定的研究

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12、

determination of the serum catalase activity in patients with liver diseases

肝病患者的血清过氧化氢酶活性测定初探

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13、

it was proved that the osazone sulfuric acid development method ( osadm) is a quite ideal dahps activity assay method, according to the osazone's absorption map, the linear relations, detecting limit.

根据糖脎的物质吸收图谱、线性关系、最小检出限等方面都证明糖脎硫酸显色法是一种比较理想的DAHPS的酶活性测定方法，并确定此法为测定酶活性的第三种方法。

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14、

for enzymatic assay, samples were dissolved in distilled water and aliquots of solution were mixed with tmb and h2o2. kinetics of enzymatic reaction was recorded at 700 nm and hrp activity was calculated in respect to the kinetic curve using fresh-prepared hrp as a control. 3.

酶活性测定时，样品用双蒸水溶解，吸取样品溶液，与TMB和H2O2混和后于700nm处测定反应反应进程曲线，以含有等量新配制HRP的反应体系为对照，计算酶的相对活性。

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15、

three detecting methods in yeast proteinase a ( pra) assay including uv absorbance, lowry method and modified bradford method were compared when using casein or hemoglobin as substrates.

其中以蛋白质为底物时的酶活性测定方法方便快捷，但是当酵母蛋白酶A酶活性较低时，产物采用紫外吸收法以及Lowry法的检测灵敏度都比较低。

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16、

the results showed that pa19, pa2 and pk1 strains had high nitrogenase activities.

经固氮酶活性测定，PA19、PA2和PK1菌株具有较强的固氮酶活性。

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17、

the assay of enzyme activity indicates that there was cam dependent ca 2+ atpase activity, but no nad kinase one.

酶活性测定实验表明在蚯蚓CaMBPs中有Ca2＋－ATPase活性，但无NAD激酶活性。

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18、

under drought stress, gene expressions of gus were found in leaves and roots of transgenic plants, gus activity showed that the two promoters had a stronger expression of activity after drought stress-induced.

在干旱胁迫处理条件下，在转基因植株的叶片和根部都发现GUS基因的表达，从GUS酶活性测定结果表明启动子都具有较强的表达活性，在干旱胁迫诱导后表达活性得到提高。

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19、

to study the protective effect of vitamin e ( ve) on hyperphosphorylation of cytoskeleton tau in neurons induced by calyculin a ( ca) and the mechanism, ve or ve plus ca was administered to the cultured slices.

通过免疫印迹，酶活性测定等方法在脑片水平研究维生素E（VE）对花萼海绵诱癌素（CA）诱导的骨架蛋白tau过度磷酸化的保护作用及机制。

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20、

conclusions: 1. we established the fluorescence substrate assays for measuring the six common mps enzymatic activity in leukocytes, with good stability, specificity and suitable for clinical application. 2.

结论1.建立了以底物荧光法为主的外周血白细胞六种常见MPS酶活性测定方法，具有良好的稳定性、特异性和敏感性，操作相对简单、快速，适合临床推广应用。

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