Differential Display of Asthma-Related Genes by Reverse-transcriptase PCR

Methods R and/ or G banding technique was used for analysis of karyotype. DNA blotting for HRX gene rearrangement, and polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT PCR) for fusion gene detection.

方法用R带和（或）G带分带技术进行核型分析，DNA印迹法检测HRX重排，聚合酶链反应和逆转录聚合酶链反应进行融合基因检测。

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Method RhD mRNA was analyzed through reverse transcriptase PCR ( RT-PCR) and cDNA sequencing in 4 individuals with different Rh phenotypes ( CcDDEe 、 CCDDee 、 CcDDee and ccDdee).

方法采用逆转录PCR（RT PCR）技术检测4名不同Rh表型（CcDDEe、CCDDee、CcDDee和ccDdee）个体的RhD mRNA，然后进行cDNA测序分析。

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Objective To establish polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT-PCR) for detecting human cytomegalovirus ( HCMV) and to find out the relationship between diabetes mellitus ( DM) and HCMV infection.

目的建立检测人巨细胞病毒（HCMV）感染的聚合酶链反应/逆转录&聚合酶链反应（PCR/RT-PCR）方法，并探讨糖尿病与HCMV感染的关系。

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After sacrificing the rats in twelve weeks, serum and aorta were studied through the techniques: biochemistry, radioimmunoassay, immuno-histochemistry ( IHC), reverse transcriptase PCR ( RT-PCR) and western blot.

各组大鼠实验12周后处死，提取血清、主动脉、通过生化、放免、免疫组化、RT&PCR法及蛋白印迹杂交法进行研究。

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Methods Total RNA in the metastatic lymph nodes from patients with colorectal cancer was extracted routinely, and reverse transcriptase PCR employed to amplify the heavy chain Fd and light chain κ cDNA.

方法取大肠癌病人转移淋巴结，提取淋巴结总RNA，逆转录PCR扩增重链Fd和K轻链cDNA。

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Extraction of total RNA from culture clones which were expanded, and then detecting the target gene was integrated into the genome of HeLa cells through reverse transcriptase PCR method ( RT-PCR).

对单克隆进行扩大培养，提取其总RNA，逆转录酶PCR法（Reverse transcriptase-PCR，RT-PCR）检测到目的基因已整合到HeLa细胞基因组中，说明重组质粒在细胞中稳定表达。

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Roles of the EnvZ/ OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR ( qRT-PCR).

同时通过突变分析、定量逆转录聚合酶链反应（qRT-PCR）研究了EnvZ/OmpR二元系统在应对渗透压力中的作用。

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One day the president of the college came to visit the Chinese teachers.

一天，学校的校长来到中国教师的住所。

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A Study on China's Teacher Policy

中国教师政策研究

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The Realization of Compliments by Chinese Teachers

中国教师表扬言语行为的实施研究

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What is more, teachers are respected in China more and more.

再者，在中国教师获得越来越多的尊重。

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The author also interview 4 students and 5 Chinese teachers.

作者通过问卷调查和采访进行研究，做了80多个西方学生的问卷，4个西方学生和5个中国教师的采访。

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A Study of the Problem Related to the Publication of Academic Papers by Chinese Teachers

中国教师发表论文问题研究

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A Chinese language learning craze around the globe has created an urgent demand for teachers from China.

全球学习汉语的热潮引起了对中国教师的急切需求。

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On the other hand, foreign teachers are preferred for classroom climate.

另一方面，学生们认为外籍教师的课堂气氛优于中国教师。

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Reform of Teacher Education and Reconstruction of Teacher Professional Identity in China

当代中国教师教育改革与教师专业身份之重建

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Chinese scholars and professors teaching at Confucius Institutes across the United States on type J-1 visas have been requested to leave the country no later than June 30.

在美国持有J-1签证的孔子学院中国教师日前得令，将不得不于6月30日离境。

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Conception Renew and Institution Construct of Teacher Training& Summary of The 1st Forum on Teacher Training in China

教师培训的理念更新与制度保障&首届中国教师培训论坛综述

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They told me they were eager to have Chinese teachers.

他们热切希望有中国教师授课。

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There also needs to be a fundamental change in the teaching approach, especially where Chinese teachers are concerned.

教学方法也需要根本变革，特别是中国教师。

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