Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.

  • 方法用RT-PCR方法从能分泌特异性抗人AFP单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
  • 来源:互联网摘选更新时间:2026-07-14 01:08:25

  • 重点词汇
  • andconj. 和;与;而且;于是;然后
  • genesn.<生>基因( gene的名词复数 );
  • methodsn.method的复数:方法;方法( method的名词复数 );方法论;教学法;分类法;
  • vh[医][=biotin]维生素h,生物素;
  • byprep. 在…旁边;表示方式;由于;经过;到…之前;
  • clonedv.(使某物)无性繁殖( clone的过去式和过去分词 );
  • monoclonal antibody单克隆抗体;
  • fromprep. (表示时间)从…;(表示原因)因为;(表示来源)来自…;(表示分离)与…分离[隔开];
  • 相关例句
1、

[ Abstract] Objective To obtain the VH and VL variable region genes of murine McAbs to human CD molecule.

目的获取系列鼠抗人CD分子单抗的轻链及重链可变区基因并进行克隆和测序。

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2、

Objective: To probe a therapy method and scheme about pigmentary lesions ( PL) and vascular lesions ( VL).

目的:探究皮肤色素性疾病和血管性疾病的治疗方法和方案。

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3、

Integrated electromyogram ( IEMG) ratio and provoke time difference of VMO and VL in two groups were compared.

比较两组运动员的股内斜肌(VMO)与股外侧肌(VL)的肌电积分比率(VMO∶VL)及其VMO和VL激发时间的差异。

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4、

Take the cDNA as a template, the VH and VL gene were amplified by PCR.

以cDNA为模板扩增VH和VL基因片段。

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5、

In recent years, virtual instrument ( VI) and virtual laboratory ( VL) have been widely used in each domain of technology.

近年来,虚拟仪器(VI)和虚拟实验室(VL)已广泛地用于科技的各个领域。

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6、

RESULTS: The VH and VL genes were homologous with the published gene sequences of mouse antibody variable region.

结果:克隆的基因序列符合小鼠轻、重链可变区基因的特征;

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7、

The introduction of γ-VL unit resulted in significant decrease of the molecular weight and melting point of the copolymers.

γVL单元的引入使得共聚物的分子量和熔点下降。

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8、

Conclusion: Results suggest that the method have better stability and easy repetition, and could get higher quantity of VH and VL.

结论:提示该方法具有稳定性好,易于重复,扩增后VH和VL量较高的优点。

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9、

The central affairs of the VL are now co-ordinated by an elected council.

VL的主要事务现由经过选举产生的委员会进行协调处理。

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10、

VL could be effectively controlled by combination of dog elimination, patient treatment and focal residual spraying with insecticide.

消灭病犬、治疗病人和在疫点用杀虫剂滞留喷洒能有效控制VL流行。

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11、

LIVAL COLOR VL colours can generally be sprayed onto the leather directly.

绒面革上色剂LIVAL COLOR VL一般可不经稀释直接用于皮革上。

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12、

The synaptic relationships of VL nucleus and cruciate cortex of the cat

猫丘脑腹外侧核与十字沟周围大脑皮层的突触联系&损毁溃变法结合电镜的研究

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13、

CONCLUSION The obtained VL gene is potentially the functional gene of the McAb against human laryngeal carcinoma.

结论该基因为抗人喉癌单克隆抗体LC9-G5轻链可变区基因。

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14、

The central Catalog pages are maintained by the Council of the VL.

中心分类页面则由VL委员会维护。

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15、

VL gene mutation of lupus ds DNA antibody may modify binding DNA affinity of antibody

狼疮ds-DNA抗体VL基因的突变可能影响抗体的致病性

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16、

VL character segmentation is to segment the license image into single characters for recognition.

车牌字符的分割是指把车牌图像分成单个的字符图像,以便进行单字符识别。

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17、

The reasons of viscosity loss ( VL) are investigated and following results are obtained.

探讨了引起粘度损失的原因,结果如下。

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18、

Study of Modeling Methods and Constructing Technologies for Web-Based Virtual Laboratory and Implementation of VL

网上虚拟实验室建模方法与构建技术研究及系统实现

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19、

VL and GAS increased activity region significantly due to cadence.

VL和GAS活动区域显著增大。

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20、

The study on VL has become a trend.

对于VL的研究已经出现快速发展的趋势。

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