The results indicated that the transformation efficiency was up to 5.5%, which was obviously higher than that of pollen tube pathway.

Since the transgenic technology of pollen tube pathway method was formally established, it has been widely used in crop genetic improvement.

中国学者创立花粉管通道法转基因技术以来，在多种农作物分子育种中得到应用。

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Transformation of Wheat with Defective Replicase Gene of BYDV-GPV via Pollen Tube Pathway

通过花粉管途径将BYDV-GPV株系缺失复制酶基因导入小麦

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Transgenic wheat mediated by pollen tube pathway

利用花粉管通道途径获得转基因小麦

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Cotton Transformation with the Pollen Tube Pathway

花粉管通道法介导的棉花遗传转化

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Application of a method for the genetic transformation in monocotyledon via the pollen tube pathway

花粉管通道法在单子叶作物遗传转化上的应用

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Study on the formation date of pollen tube pathway on upland cotton

陆地棉花粉管通道形成时期的研究

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Variant germplasm obtained by pollen tube pathway technique and SSR analysis

利用花粉管通道技术创造棉花变异种质及其SSR标记分析

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The Development of Transgenic Technique by Pollen Tube Pathway

花粉管通道转基因技术研究进展

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Research on soybean pollen tube pathway was carried out.

对大豆花粉管通道技术进行深入研究。

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Pollen Tube Pathway Transgenic Technique and Application in Wheat Molecular Breeding

花粉管通道转基因技术及在小麦分子育种中的应用

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Transgenic wheat resistant to Barley Yellow Dwarf Virus obtained via pollen tube pathway

花粉管通道法介导的抗大麦黄矮病毒转基因小麦研究

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Japonica rice cv77170 was transformed with pBI222 carrying hygromycin phosphotransferase gene by using biolistic bombardment and fertile transgenic rice plants were obtained.

基因枪转化将潮霉素磷酸转移酶基因导入粳稻品种77170，获得可育的转基因植株，研究外源基因遗传的稳定性。

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The soybean ferritin gene can be transferred into upland rice using the method of Biolistic and Agrobacterium-mediated transformation, which increased iron concentration in the transgenic plant seeds.

利用基因枪和农杆菌转化法可将大豆铁结合蛋白基因导入旱稻基因组中，并使转基因稻米铁含量增高。

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Microinjection, retrovirus mediated method and embryonic stem cells method were all the traditional methods of producing transgenic animals. But these methods had defects and limited application in the research of transgenic animals in the future.

传统的制作转基因动物方法有显微注射法、逆转录病毒感染法和胚胎干细胞法等，但每种方法都有其缺陷，限制了其在今后转基因动物研究中的广泛应用。

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Study on Techniques of Making Transgenic Mice by Microinjection Method

显微注射法制备转基因小鼠的技术研究

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METHODS: Transgenic mice were produced by pronu-clei microinjection method.

方法：原核显微注射法产生转基因小鼠。

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Furthermore, with only a half dose of DNA in the current system, by the horizontal system under × 100 magnification both the survival rate of pronuclear embryos following microinjection and the integration frequency of exogenous DNA injected into embryos also increased.

此外，利用该系统还可在低倍镜下（×100）将DNA常规注射量减半注射，提高了原核胚注射后的成活率，其注射胚的外源基因整合率亦明显提高。

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To the great extent, the study and application of transgenic animal are restricted by the inherent limitation of pronuclear microinjection.

显微注射技术本身固有的缺点在很大程度上限制了转基因动物的研究及应用。

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The Effect of Pronuclear Microinjection with Foreign DNA and Culture Conditions on the Development in vitro of Mouse Pronuclear Embryos

影响小鼠转基因原核胚体外发育的若干因素

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The basic reliable methods available to produce transgenic animal such as pronuclear microinjection has limited application by low efficiency and mosaic integration into the genome.

由于生产转基因动物的基本方法如原核注射法等普遍存在外源基因整合的低效性和随机性等问题，因而在应用中受到很大制约。

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