Variant germplasm obtained by pollen tube pathway technique and SSR analysis

The Development of Transgenic Technique by Pollen Tube Pathway

花粉管通道转基因技术研究进展

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Research on soybean pollen tube pathway was carried out.

对大豆花粉管通道技术进行深入研究。

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Pollen Tube Pathway Transgenic Technique and Application in Wheat Molecular Breeding

花粉管通道转基因技术及在小麦分子育种中的应用

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Transgenic wheat resistant to Barley Yellow Dwarf Virus obtained via pollen tube pathway

花粉管通道法介导的抗大麦黄矮病毒转基因小麦研究

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Japonica rice cv77170 was transformed with pBI222 carrying hygromycin phosphotransferase gene by using biolistic bombardment and fertile transgenic rice plants were obtained.

基因枪转化将潮霉素磷酸转移酶基因导入粳稻品种77170，获得可育的转基因植株，研究外源基因遗传的稳定性。

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The soybean ferritin gene can be transferred into upland rice using the method of Biolistic and Agrobacterium-mediated transformation, which increased iron concentration in the transgenic plant seeds.

利用基因枪和农杆菌转化法可将大豆铁结合蛋白基因导入旱稻基因组中，并使转基因稻米铁含量增高。

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Microinjection, retrovirus mediated method and embryonic stem cells method were all the traditional methods of producing transgenic animals. But these methods had defects and limited application in the research of transgenic animals in the future.

传统的制作转基因动物方法有显微注射法、逆转录病毒感染法和胚胎干细胞法等，但每种方法都有其缺陷，限制了其在今后转基因动物研究中的广泛应用。

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Study on Techniques of Making Transgenic Mice by Microinjection Method

显微注射法制备转基因小鼠的技术研究

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METHODS: Transgenic mice were produced by pronu-clei microinjection method.

方法：原核显微注射法产生转基因小鼠。

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Furthermore, with only a half dose of DNA in the current system, by the horizontal system under × 100 magnification both the survival rate of pronuclear embryos following microinjection and the integration frequency of exogenous DNA injected into embryos also increased.

此外，利用该系统还可在低倍镜下（×100）将DNA常规注射量减半注射，提高了原核胚注射后的成活率，其注射胚的外源基因整合率亦明显提高。

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To the great extent, the study and application of transgenic animal are restricted by the inherent limitation of pronuclear microinjection.

显微注射技术本身固有的缺点在很大程度上限制了转基因动物的研究及应用。

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The Effect of Pronuclear Microinjection with Foreign DNA and Culture Conditions on the Development in vitro of Mouse Pronuclear Embryos

影响小鼠转基因原核胚体外发育的若干因素

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The basic reliable methods available to produce transgenic animal such as pronuclear microinjection has limited application by low efficiency and mosaic integration into the genome.

由于生产转基因动物的基本方法如原核注射法等普遍存在外源基因整合的低效性和随机性等问题，因而在应用中受到很大制约。

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Five transgenic positive mice were obtained through pronuclear microinjection.

通过原核注射，获得5只转基因阳性鼠；

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With the combination of gene targeting, animal transgenic and animal cloning technologies, there have been a series of theoretical and applied research of animal genomes modified, such as fixed-point gene repair, the treatment of genetic defects and inactivation of animal unfavorable gene.

利用动物转基因技术和动物克隆技术，再结合基因打靶技术，人们开展了基因的定点修复、遗传缺陷的治疗、动物不利基因的敲除失活等一系列的动物基因组修饰的理论和应用研究。

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Thus, the orf 144b gene required for the multiple-DNA-damaging agent resistance of Dr was designated with the name of pprA ( Pleiotropic gene promoting DNA repair).

在抗辐射菌中，这种对多种DNA损伤剂产生抗性所需要的基因（orf144b），我们命名为pprA（Pleiotropic gene promoting DNA repair）基因。

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Histone lysine methylation has important functions in many biological processes that include gene expression, X-chromosome inactivation, gene imprinting and DNA repair.

组蛋白赖氨酸甲基化作为共价修饰的一种在基因表达调控、X染色体失活、基因组印迹、DNA修补等生物学过程中起重要作用。

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Objective: To express the influences of E Jiao on 6 kinds interrelated gene expression during bone repair, understand E Jiao regulation target, and create gene theory of Traditional Chinese Medicine.

目的：揭示阿胶在骨愈合过程中对6种相关基因表达的干预作用，了解阿胶的调节靶点，探索建构中药基因组学的途径。

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The expression of p53 gene that induces the repair of DNA damage or apoptosis depends on the different model of cerebral ischemia and the different moment and region of observed brain tissue.

缺血模型、时间点及观察部位的不同，导致基因表达调控机制的差异，这可能决定着p53的表达最终是诱导DNA损伤修复还是导致细胞凋亡。

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Objective: To establish an experimental mandibular defect animal model and provide a template in gene therapy for the repair of mandibular defects.

目的：建立用于研究下颌骨骨缺损基因治疗的实验动物模型及基因导入方法。

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