The rats of group B were treated with EPO after globe ischemia-reperfusion. Brain tissues were taken out after execution at different time point. The expression of caspase-9 mRNA was detected with reverse transcription PCR ( RT-PCR) technique.

Methods Techniques of cell culture in vitro and RT PCR ( reverse transcription PCR) were used, gene expressions of p53 and fas in 4 tumor cell lines were measured after treated with ethanol extractive and water extractive.

方法采用肿瘤细胞体外培养技术及RT-PCR技术，观察阿魏菇不同剂量的水提物及醇提物对体外培养的四种肿瘤细胞p53及fas基因表达的影响。

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The method of reverse transcription PCR ( RT-PCR) is more sensitive and reliable than that of enzyme-linked immunosorbent assay on nitrocellulose membranes ( NCM-ELISA).

对于甘薯病毒病的检测，反转录PCR法（RT-PCR）比硝化纤维素膜酶联免疫吸附检测法（NCM-ELISA）更灵敏和可靠。

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Methods The interested segment was obtained by reverse transcription PCR with the designed specific primers, and inserted into pMD-18T vector by T/ A match.

方法设计特异引物，用RT-PCR方法从乳腺癌组织扩增获得目的片段，利用T/A克隆将PCR产物插入pMD-18T载体；

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Recombinant human EGF was used to stimulate HepG_2 cells and semi-quantitative reverse transcription PCR was adopted to detect the expression of VEGF in HepG_2 cells.

离体实验中，用重组人EGF刺激人肝癌细胞系HepG2，采用半定量逆转录PCR检测VEGF的表达情况。

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The silencing effects of TIMP-1 on the mRNA and protein levels were examined by reverse transcription PCR ( RTPCR) and Western blot analysis respectively.

采用RT-PCR和Western印迹测定宿主细胞TIMP-1在基因和蛋白水平的沉默效果。

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Reverse transcription PCR and in situ hybridization were used to detect the expression changes of CaBP mRNA in the brain of PD and control mice.

用反转录PCR及原位分子杂交方法检测PD鼠和正常鼠脑内CaBP mRNA的表达变化。

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Real-time fluorescence quantitative reverse transcription PCR in determination of guanylyl cyclase-C gene expression in peripheral blood of patients with colorectal cancer and its clinical significance

实时荧光定量PCR检测大肠癌患者外周血鸟苷酰环化酶C基因及其临床意义

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A Reverse Transcription PCR that Distinguishes the Isolates of Low Virulent Strains From Virulent Strains of Newcastle Disease Virus

鸡新城疫病毒强弱毒株RT-PCR鉴别诊断方法

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A coupled one-step reverse transcription PCR procedure for cloning the human augmenter of liver regeneration gene

RT-PCR一步法克隆人肝再生增强因子基因

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A reverse transcription PCR system was established for the detection of Noroviruses in shellfish.

本文建立了贝类产品中诺沃克病毒检测的普通RT-PCR方法。

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Identification of Viruses Causing Respiratory Tract Infection by Single-tube Multiplex Reverse Transcription PCR

应用多重反转录PCR技术检测病毒性呼吸道感染

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CDNA-AFLP differential patterns for 5 selected genes were confirmed via real-time reverse transcription PCR analysis.

用荧光实时定量PCR分析法证实了cDNA-AFLP基因表达结果。

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Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR

实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型

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Application of reverse transcription PCR in the detection of respiratory syncytial virus infection

反转录-聚合酶链反应在呼吸道合胞病毒感染检测的应用

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Application of in situ reverse transcription PCR techniques

原位反转录PCR技术的应用

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Detection of Hepatitis A Virus by Two Reverse Transcription PCR Approaches

两种反转录聚合酶链反应检测甲型肝炎病毒

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Methods: By using RT PCR method, the expression of hematopoietic growth factor in placenta was assessed.

方法：通过RT-PCR方法检测胎盘组织中造血相关因子的表达情况。

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Transfected COS 7 cells were identified by RT PCR after routine cultivation.

取常规培养的细胞进行RT PCR鉴定。

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Detection of-Sugarcane mosaic virus-by dot hybridization and RT PCR technique

利用斑点杂交法和RT-PCR技术检测甘蔗花叶病毒

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The bFGF mRNA expression was quantitated by RT PCR assay.

RT PCR检测细胞碱性纤维化生长因子mRNA的表达。

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