Experimental Studies on Anti-HSV-2 Effect of Purslane Misture Water Extract

The cultured cells were collected at the first, third, 5th, and 7th days of post-infection. The expression of HLA class II antigen of the infected monocytes was measured by alkaline phosphotase anti-alkaline phosphotase method ( APAAP).

方法用单纯疱疹病毒2型（333株）感染人单核细胞，于感染后1、3、5、7d分别收集细胞，用APAAP法检测单核细胞HLA-DR、HLA-DQ表达水平。

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RESULTS: We have cloned full-length gG-2 gene of HSV-2.By making an analysis to B cell epitope of gG-2, we found that the homology in N-terminal of gG-1 and gG-2 genes of HSV was very low.

结果：完成单纯疱疹病毒2型（HSV-2）gG-2全长基因克隆.通过B细胞抗原表位分析，发现HSV-1的gG-1和HSV-2的gG-2氨基端的同源性很低。

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Objective To explore the relationship between cervical erosion and HSV-2 infection and the treatment with interferon suppository in STD clinics.

目的探讨性病门诊妇女宫颈糜烂与单纯疱疹病毒2型（HSV2）的活动感染的关系，并初步探讨用干扰素栓治疗的临床效果。

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Conclusion HLA class ⅱ antigen expression of monocytes was inhibited by HSV-2 infection at early stage, which may be one of means of virus escaping host immunity.

结论单纯疱疹病毒2型感染早期能抑制单核细胞表达HLA-Ⅱ类抗原，HSV-2抑制单核细胞表达HLA-II类抗原可能是HSV-2逃避机体的手段之一。

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Methods 100 specimens of NGU and 30 control specimens were detected for virus isolation using cell culture combining immunofluorescence method.

方法用细胞培养法和免疫荧光法联合检测100例非淋菌性尿道炎（NGU）组和30例对照组患者尿道分泌物标本单纯疱疹病毒2型（HSV2）。

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Effect of Herpes Simplex Virus-2 Infection on the HLA Class II Antigen Expression of Human Monocytes

单纯疱疹病毒2型对单核细胞表达HLA-II类抗原的影响

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To construct Pichia pastoris expression vector of herpes simplex virus type 2 ( HSV-2) envelope glycoprotein D mature peptide gene, then analyze the gene sequences for expressing the recombinant gD.

构建单纯疱疹病毒2型包膜糖蛋白D成熟肽基因毕赤酵母表达载体，并对序列进行分析，为进行高抗原性的真核表达重组gD蛋白奠定基础。

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Herpes simplex virus type 2 ( HSV-2) is a ubiquitous human pathogen mainly causing genital herpes ( GH).

单纯疱疹病毒2型（HSV-2）是一种常见的人类至病菌，感染人腰部以下的皮肤和粘膜而主要引起生殖器疱疹。

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Herpes simplex virus type 2 ( HSV-2) is a linear, double stranded DNA virus, HSV-2 is the primary cause of genital herpes.

单纯疱疹病毒2型（HSV-2）是线性双链DNA病毒，是引起生殖器疱疹（GH）的主要病原体。

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Detecting and meaning about antigen of herpes simplex virus type 2 ( HSV-2) in cervical tissue

宫颈活检组织中单纯疱疹病毒2型（HSV-2）抗原的检测意义

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Serum HSV-2 IgG antibody was determined by ELISA in 80 outpatients with STD ( gonorrhea 14, NSGI 38 and condyloma accuminatum 28).

采用ELISA技术检测了80例STD门诊患者（其中淋病14例、非特异性泌尿生殖道感染38例及尖锐湿疣28例）血清单纯疱疹病毒2型（HSV－2）IgG抗体。

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To develop a real-time PCR method for herpes simplex virus-2 ( HSV-2).

目的建立一种快速、特异、准确定量的单纯疱疹病毒2型（HSV-2）检测方法。

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Objective: To clone and express recombinant HSV-2 antigen in E. coli.

目的：在大肠杆菌中表达重组单纯疱疹病毒2型（HSV-2）抗原。

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Comparison of Four Commercially Available ELISA Kits for Detecting HSV-2 Type-specific IgG Antibodies

四种商品化单纯疱疹病毒2型特异性IgG抗体检测试剂盒的比较

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Objective: To clone and express immunodominant fragment of glycoprotein G of HSV-2 ( FgG-2).

目的：通过基因工程技术，克隆和表达单纯疱疹病毒2型糖蛋白G（HSV-2gG）基因的免疫优势表位片段。

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Gene cloning and B cell antigen epitope analysis of HSV-2 glycoprotein G

单纯疱疹病毒2型糖蛋白G的基因扩增、克隆及其B细胞抗原表位分析

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Prophylactic Effects of a HSV-2 DNA Vaccine in Guinea Pigs

单纯疱疹病毒2型DNA疫苗对豚鼠抗病毒感染的研究

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HSV-2 is a member of the herpesvirus family, and is the leading cause of genital herpes worldwide.

单纯疱疹病毒2型是成员之一，疱疹病毒家族，是导致生殖器疱疹世界各地。

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Beijing-7 strains of varicella-zoster virus were inoculated in human embryo lung diploid cells which were in logarithmic growth phase. When CPE appeared in the cells infected with VZV, pre-existent maintenance media was exchanged by MEM which contained no bovine serum.

选择处于对数生长期的人胎肺二倍体细胞（2BS），接种北京-7株水痘-带状疱疹病毒.待受染病毒的细胞出现CPE时，将维持液换为不含牛血清的MEM。

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Objective To understand the prevalent status of varicella-zoster virus in the population of Shenzhen City and provide theoretic basis for the prevention of Varicella-zoster.

目的了解深圳市人群水痘-带状疱疹病毒（VZV）的流行现状，为制定水痘防制措施提供依据。

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