aane

Result: The concentration of TNF α increased after keratinocytes were stimulated by AANE for 12 hours or longer time.

结果：槟榔提取物干预角朊细胞12h后肿瘤坏死因子α的量开始增加；

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Method: Human oral mucosa keratinocytes were isolated, cultured, stimulated with AANE, The supernatant was aspirated and the level of TNF α was detected using radioimmunoassay.

方法：通过体外培养口腔黏膜角朊细胞，用不同浓度槟榔提取物对其进行干预，放射免疫方法检测不同干预时段培养上清中的肿瘤坏死因子α浓度。

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The effection of mast cells ( MC) on collagen metabolism of oral submucous fibrosis ( OSF) induced by aqueous areca nut extracts ( AANE) in rats was studied by transmission electron microscope, histochemical and biochemical analyses.

借助组织化学、生化分析和透射电镜方法，探讨肥大细胞对水溶性槟榔提取液诱发的大鼠口腔粘膜下纤维性变（OSF）的胶原代谢的影响。

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Method: Stimulated the endothelial cells with aqueous areca nut extract ( AANE) and the cultured medium was aspirated. Then the level of NO produced by endothelial cells was detected by method of ELISA.

方法：用槟榔提取物干预体外培养的内皮细胞，收集培养上清，用ELISA方法检测内皮细胞分泌一氧化氮（NO）的浓度。

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Objective: To explore the pathogenesis of oral submucous fibrosis ( OSF) by observing the effect of Aqueous areca nut extract ( AANE) on the level of tumor necrosis factor α( TNF α) produced by oral keratinocytes.

目的：观察槟榔提取物对口腔黏膜角朊细胞分泌肿瘤坏死因子α的影响，探讨槟榔诱发口腔黏膜下纤维性变的机制。

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The purpose of this study was to establish the animal model of oral submucous fibrosis ( OSF) in buccal mucosa of SD rats by submucosa injecting and surface painting aqueous areca nut extracts ( AANE).

采用粘膜下注射及表面涂布水溶性槟榔提取液（AANE）相结合方法，诱发SD大鼠颊部口腔粘膜下纤维性变（OSF）。

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Result: The level of NO produced by endothelial cells was inhibited by AANE.

结果：槟榔提取物能抑制体外培养的内皮细胞分泌NO。

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