VTT

VTT also laccases intercomparsion reaction catalyzed by enzymes, non-ferrous products of indicator for leakage.

VTT也 利用漆酶催化促酶反应形成有色产物,研究渗漏指示剂的构成.

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Moreover, VTT is much less virulent than vaccinia WR but remains neurovirulent in mice and causes significant body weight loss after intranasal inoculation.

体内实验显示，尽管天坛株比神经毒性的WR株毒性弱很多，但天坛株仍保留相当的神经毒性，而且经鼻腔接种可引起小鼠体重严重下降。

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The VTT Bank Card System

VTT银行磁卡系统

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We have previously characterized the phenotypic properties of VTT both in vitro and in vivo and have identified its virulence property in causing the body weight loss in mice once inoculated directly through the intracranial route.

之前我们已经鉴定了野生型天坛株在体内体外的生物学特性，并且已经通过检测鼻腔途径导致的体重降低和直接颅腔接种鉴定了其毒力。

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In addition, VTT produced severe cytopathic effects ( CPE) in nearly all the tested cells, including 4 human cell lines.

天坛株在几乎所有测试的细胞中均能产生严重的至细胞病变效应（cytopathic effects，CPE），包括四种人类来源的细胞系。

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To explore the potential use of VTT as a live vaccine vector, we have generated three modified VTT strains ( MVTTs) by homologous recombination with the green fluorescent protein ( GFP) gene at three distinct genomic locations.

为了开发天坛株作为活载体来构建疫苗，我们通过同源重组的方法将绿色荧光蛋白基因GFP导入到其基因组的不同位置，构建了三种改良的痘苗病毒天坛株。

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Furthermore, diminished CPE and/ or reduced cell-to-cell spread of the modified viruses occurred in most of the cells tested including human-derived cell lines compared with its parental VTT strain.

在多数动物细胞中包括在人源细胞中重组病毒具有比野生天坛株病毒更低的细胞到细胞间的扩散水平或更小的对细胞的毒性。

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We found that VTT has broad host cell range that 11 of the 12 mammalian cell lines studied and primary CEF are permissive to VTT infection whereas one, CHO-K1, is non-permissive.

我们发现天坛株具有广泛的宿主范围，经研究的12种哺乳动物细胞中的11种及原代鸡胚细胞CEF均是天坛株的允许细胞，而中国仓鼠卵巢细胞CHO-K1是天坛株的非允许细胞。

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