PCR

Results The structures of recombinant plasmids were comfinaed by PCR and sequencing.

结果重组体经PCR和测序证实准确连接.

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The construct was confirmed by PCR, restriction enzyme digestion and direct sequencing.

经限制性内切酶酶切和测序证实。

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The expressions of bcl-x, bax and eNOS were measured by SQ-RT-PCR technique.

采用半定量逆转录聚合酶链反应(SQ-RT-PCR)法检测内皮细胞bcl-x 、 bax以及 eNOS表达.

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Expression of TGF � � 1 mRNA was evaluated by RT PCR.

RT?PCR方法测定肺组织内TGF?β1的表达.

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Genotyping of hepatitis E virus by PCR combining with single restriction endonuclease analysis

PCR结合单酶切对戊型肝炎病毒进行基因分型的研究

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Results CnA α, CnA � � could be detected by RT-PCR but no CnA � �.

结果RT -PCR 可检测到CnAα 、 CnAβ,未检测到CnAγ.

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Methods PCR was performed to amplify the AMG encoding region.

方法　采用PCR技术体外扩增AMG完整分泌肽编码区.

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The mutation was confirmed by digestion of the PCR products with restriction enzyme.

发现突变后用特异性限制性内切酶对突变位点进行酶切，验证突变。

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REP-PCR , AFLP technique can reveal the genetic differentiation properly.

AFLP和 REP -PCR 技术均能很好地反映菌株的遗传学差异.

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And have been identified by PCR and restriction enzyme digestion successfully constructed recombinant viruses.

并经过PCR和酶切鉴定重组病毒构建成功。

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Methods Polymerase chain reaction ( PCR ) connected with reverse dot blot ( RDB ) were performed.

方法采用多聚酶链反应 ( PCR ) 结合反向斑点杂交 ( RDB ) 技术.

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The classification of integrons was performed by analyzing the positive PCR products using restriction fragment length polymorphism ( RFLP).

PCR-限制片段长度多态性（RFLP）筛查整合子并分类；

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Detection of Hypodermal and Hematopoietic Necrosis Baculovirus of Penaeid Shrimp by One-tube Multiplex PCR Method

多重PCR法检测对虾皮下和造血器官坏死杆状病毒

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EST-PCR and EST-SSR marker were developed in Chinese cabbage.

建立了白菜的EST -PCR 和EST-SSR标记.

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The mutation was confirmed by allele specific PCR ( ASPCR ).

应用等位基因特异性 PCR 验证测序所发现的突变.

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The ISOObp promoter of PNZIP gene was cloned by adaptor PCR method.

因此,我们利用接头PCR技术克隆了PNZIP基因的启动子.

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To verified the by restriction enzyme digestion, bacterial colony PCR, and sequencing analysis.

并对重组质粒进行双酶切、菌落PCR及测序分析进行鉴定。

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RT-PCR was used to detect the eNOS mRNA and iNOS mRNA.

RT -PCR 分别检测内皮细胞eNOS、iNOS基因的相对表达量.

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Searching and cloning of differentially expressed genes in liver tumor by restriction fragment differential display PCR

限制性酶切片段差异显示技术搜寻和克隆肝癌相关基因

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The comparision of different methods of DNA extracting applied in PCR detection of hypodermal and hematopoietic necrosis baculovirus

用于PCR检测对虾皮下及造血组织坏死杆状病毒（HHNBV）的一种快速提取DNA方法

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Detection of White Spot Syndrome Virus and Infectious Hypodermal and Haematopoietic Necrosis Virus of Shrimps by Mutiplex PCR

多重PCR检测对虾白斑综合症病毒和传染性皮下组织坏死病毒

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Methods DNA was extracted from peripheral blood by rapid salt method and a PCR SSP based method was introduced to analyse the genotypes of ABO blood group and its secretor status.

方法用快速盐析法提取外周血标本中的DNA，用PCR SSP扩增ABO血型、HAB分泌型等位基因。

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Analyses of PCR products in 82 HCV RNA positive Serra by restriction fragment length polymorphism

82例HCV RNA阳性血清聚合酶链反应产物的酶切分析

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Recombinants were confirmed by colony PCR and restriction enzyme digestion.

经菌落PCR和质粒酶切鉴定后，将目的基因进行序列测定。

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The genotypes of apoE, apoC-I and LRP genes were analyzed by using PCR and restriction fragment length polymorphism ( RFLP) techniques.

应用PCR-RFLP技术，对apoE、apoC-I与LRP基因进行分析。

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PCR were using to detect bla ( TEM ), bla ( SHV ) and bla ( CTX-M ) genes.

应用聚合酶链反应(PCR)方法扩增产ESBLs株的bla ( TEM ) 、 bla ( SHV ) 和bla ( CTXM ) 基因.

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Methods: Polymer-ase chain reaction ( PCR ) was used to determine the ACE genotype.

方法: 采用聚合酶链反应 ( PCR ) 方法检测ACE基因型.

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RT-PCR was used to determine GK and PEPCKgene expression ( corrected by � �-actin ) .

用RT -PCR 检测GK和 PEPCK mRNA表达水平的变化.

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The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing.

通过酶切、PCR及测序鉴定各重组体。

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Histamine-producing strains could be detected by PCR test or DNA probe technology.

利用PCR与DNA探针技术能够快速检测葡萄酒中的组胺产生菌.

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