HFF

Objective The experiment was carried out to study the effects of EGF and HFF in culture medium on in vitro maturation. fertilization and embryo developmental competence of human immatured oocytes. Mothods Human immatured oocytes came from patients with PCOS who were received IVM treatment.

目的探讨培养液中添加人成熟卵泡液（HFF）、表皮生长因子（EGF）对人未成熟卵母细胞体外成熟（IVM）、体外受精（IVF）及其胚胎发育的影响。

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Methods 84 Wistar rats were used to establish experimental DM rats model by high fat forage ( HFF) and injection of 40 mg/ kg STZ.

方法84只Wistar大鼠，采用高脂饲料饲养和注射40mg/Kg STZ方法建立实验性糖尿病动物模型。

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The purity of P3 passage HFF was more than 95%.

P3代细胞波形蛋白染色显示成纤维细胞纯度达到95%以上。

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Results After 2 weeks of GLP-1 analog injection, thymus index of DM and all therapeutic group was lower than that in normal control group and HFF group ( P < 0.01), but there were no significant difference in spleen index among all groups ( P> 0.05);

结果GLP-1类似物治疗2w后，糖尿病模型组和各治疗组与正常对照组和高脂饲料组相比较胸腺指数存在显著性差异（P<0.01）；各组动物的脾指数之间没有显著性差异（P>0.05）。

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Conclusion: It is an effective method to cultivate HFF cells with high productivity and survival rate.

结论：该方法可获得高产量、高活力的HFF细胞，是一种可靠的HFF细胞培养方法。

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Objective To evaluate the effect of human follicular fluid ( HFF) on mouse embryo culture.

目的探讨人卵泡液对小鼠胚胎生长发育的影响。

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