EAEA

Detection of the eaeA gene in Escherichia coli isolated from children with diarrhoea and characterization of the strains possessing the eaeA gene

腹泻儿童粪便分离的大肠杆菌中eaeA基因的检测及携带eaeA菌株的特性研究

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Methods: Seven genes of stx 1, stx 2, hly, eaeA , uidA , rfbE , fliC from different strains were detected by PCR and multiplex PCR.

方法: 利用单一PCR和多重聚合酶链反应(mPCR) 检测不同来源菌株志贺样毒素(stx1和stx2) 、 溶血素( hly )、粘附抹平因子(eaeA) 、 β-葡糖醛酸糖苷酶(uidA )、O157抗原编码(rfbE) 、 H7鞭毛抗原编码(fliC)基因.

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Results The overall virulence gene prevalence was 62.7% ( 42/ 67); and the main virulence gene type was slt_1+ slt_2+ eaeA+ hly.

结果62.7%的株菌（42/67）携带有毒力基因，毒力图谱类型主要为slt1+slt2+eaeA+hly。

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All eaeA positive isolates were rhamnose positive.

生化试验表明eaeA阳性 的大肠杆菌为乳糖发酵试验弱阳性的菌株.

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This indicates that eaeA gene is not limited to EPEC and EHEC, that the eaeA harbouring E. coli are heterogeneous with respect to their virulence determinants.

提示eaeA基因的分布不仅限于EPEC和EHEC，而且携带eaeA基因菌株含有多种毒力决定因子。

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EAEA is one of the intelligence optimization algorithms. In EAEA, the estimation of distribution algorithm and elite reservation are embedded into AEA to generate a comparison population for iteration needs.

EAEA是一种智能优化算法，它将分布估计算法和精英保留策略加入到AEA中，用于产生候选参数的对比种群。

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