CEVd

Preliminary study on One-step RT-PCR detection of Citrus exocortis viroid ( CEVd) and its distribution in different parts of sweet orange

柑橘裂皮病类病毒的一步RT-PCR法检测及其在甜橙体内的分布

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Preliminary study on comparison of Fast Micro-extraction method and SDS-KAc method, detecting CEVd in Etrog citron with symptoms by One-step RT-PCR, revealed that SDS-KAc method was preferable for eliminating non-specific band.

分别采用快速微量核酸提取法和SDS-KAc抽提法在表现症状的Etrog香橼中提取核酸，采用一步RT-PCR法对CEVd进行检测，结果显示SDS-KAc法可以消除带毒样品中非特异性条带。

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Simultaneous detection of Citrus tristeza virus ( CTV), Citrus exocortis viroid ( CEVd) and Citrus tatter leaf virus ( CTLV) by multiplex RT-PCR

柑橘衰退病、裂皮病和碎叶病的多重RT-PCR检测方法研究

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Quick Detection of Citrus exocortis viroid ( CEVd) by One-step RT-PCR

利用一步法RT-PCR检测柑橘裂皮病类病毒

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To optimize the multiplex RT-PCR, the concentration of primers and annealing temperature were repeatedly tried. Final results showed that a multiplex RT-PCR system to simultaneously detect HLB, CEVd and CTV was firstly established successfully.

就影响多重PCR的主要因素引物浓度和退火温度进行了一系列优化，建立了能同时检测HLB、CEVd、CTV的多重RT-PCR技术体系。

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The results indicated that the Chinese isolate of CEVd is different in left-terminal region from the CEVd-A strain.

扩增结果说明：CEVd中国分离物在左端T区与CEVd-A株存在差异。

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The result showed that efficacy of elimination of huanglongbing ( CHLB) BLO, CTV and CEVd by STG was 100%, 80.6% and 54.1%, respectively.

结果表明：黄龙病、衰退病、裂皮病平均脱除率分别为100%、80.6%和54.1%。

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