BCIP

Positive signals were identified by observing the blue and purple spots produced by the catalytic reaction of Alkaline phosphatase to NBT and BCIP.

通过观察碱性磷酸酶对NBT和BCIP的催化反应产生蓝紫色斑点来判定阳性信号。

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Using an NBT/ BCIP color detection system, dark purple grains were seen around cells by light microscopy and indicated stable binding between DIG-WSSV and hemocytes.

以NBT/BCIP为酶反应显色底物观察到在细胞周围形成许多暗紫色颗粒，证实病毒与细胞间存在着稳定的结合。

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Subsequently, the second signals showed bluish violet granules after incubation with SA AP and NBT/ BCIP used as the substrate.

继之以SA AP孵育后以NBT/BCIP底物显色，杂交信号呈蓝紫色颗粒。

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