1、

Methods: Total RNA of new born rat liver was extracted as a template, first strand of cDNA was synthesized by reverse transcription with olig ( dT)-primer and then amplified by PCR with the primers designed by ourselves.

方法:提取新生大鼠肝脏总RNA为模板,以寡聚dT为引物逆转录合成第一链cDNA,再以我们设计的PCR引物进行PCR扩增获取大鼠肝再生增强因子编码区双链cDNA;

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2、

The standard curves were made by real-time quantitative PCR reaction using the dilutions of RNA as the template.

将RNA标准品梯度稀释作为模板进行实时荧光定量PCR反应,制作标准曲线。

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3、

The relationship between template activity and structural variation of recombinant plasmid DNA was studied in a cell-free system for in vitro transcription which was developed with maize RNA polymerase B and recombinant plasmid DNA.

本文以重组质粒pZmS51DNA为转录模板,在无细胞离体转录系统中研究了质粒DNA的构型变化与其模板活性的关系。

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4、

Then, RT-PCR was carried out for IL-10 gene screening using total RNA as a template, specific primers which derived from GenBank data of hIL-10 as a primer for reverse transcription and PCR primers.

以总RNA为模板,根据人IL-10在GenBank中的全长开放读框设计的特异性引物进行反转录,RT-PCR进行IL-10基因筛选。PCR产物经酶切分析和DNA序列测定后;

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5、

Telomerase activity of BEL-7404 human hepatoma cells was inhibited effectively by antisense oligonucleotides against template region of telomerase RNA component, whereas no effects of inhibition were observed in control group.

端粒酶RNA组分模板区的反义寡核苷酸可以有效抑制BEL-7404人肝癌细胞的端粒酶活性,而正义和错义寡聚物则对端粒酶活性几乎没有影响。

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6、

Objective: Telomerase is a ribonucleoprotein complex that synthesizes telomeric DNA onto chromosome ends using its RNA component as template, enabling extension of cell lifespan and even immortalization.

目的:端粒酶是一种核糖核蛋白复合物,能以自身RNA成分为模板催化染色体末端端粒重复序列的延伸,使细胞得以持续增殖甚至永生化。

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7、

The sliding clamp model of transcription processivity suggests the formation of a highly stable elongation complex ( EC), in which RNA polymerase is tightly bound to the nascent transcript and template forming the characteristic transcription "bubble".

在RNA聚合酶Ⅱ介导的转录延伸过程中,其和模板DNA及转录产物RNA紧密结合,形成一个非常稳定的延伸三维复合物(elongationcomplex,EC)。

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8、

Messenger RNA ( mRNA ) -- an RNA molecule that serves as a template for protein synthesis.

信使 核糖核酸 -- 核糖核酸的一种,当蛋白质合成中当作模板指定讯息的转译.

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