1、

The basic reliable methods available to produce transgenic animal such as pronuclear microinjection has limited application by low efficiency and mosaic integration into the genome.

由于生产转基因动物的基本方法如原核注射法等普遍存在外源基因整合的低效性和随机性等问题,因而在应用中受到很大制约。

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3、

To the great extent, the study and application of transgenic animal are restricted by the inherent limitation of pronuclear microinjection.

显微注射技术本身固有的缺点在很大程度上限制了转基因动物的研究及应用。

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4、

Furthermore, with only a half dose of DNA in the current system, by the horizontal system under × 100 magnification both the survival rate of pronuclear embryos following microinjection and the integration frequency of exogenous DNA injected into embryos also increased.

此外,利用该系统还可在低倍镜下(×100)将DNA常规注射量减半注射,提高了原核胚注射后的成活率,其注射胚的外源基因整合率亦明显提高。

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5、

METHODS: Transgenic mice were produced by pronu-clei microinjection method.

方法:原核显微注射法产生转基因小鼠。

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6、

Study on Techniques of Making Transgenic Mice by Microinjection Method

显微注射法制备转基因小鼠的技术研究

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7、

Microinjection, retrovirus mediated method and embryonic stem cells method were all the traditional methods of producing transgenic animals. But these methods had defects and limited application in the research of transgenic animals in the future.

传统的制作转基因动物方法有显微注射法、逆转录病毒感染法和胚胎干细胞法等,但每种方法都有其缺陷,限制了其在今后转基因动物研究中的广泛应用。

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8、

An ion current expression after intracellular microinjection of mRNA in the Xenopus oocytes

卵胞内微注射mRNA后胞膜上出现的新离子流

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9、

Effect of microinjection of etorphine into dorsal hippocampus on forelimb withdraw reaction induced by stimulating splanchnic nerve in conscious rabbit

海马背部微量注射埃托啡对家兔内脏大神经伤害性刺激引起回缩反应的影响

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10、

Effect of intracerebroventricular microinjection of caerulein on gastric juice secretion and content of PGE_2 in gastric juice of rats

侧脑室内微量注射雨蛙肽对大鼠胃液分泌及胃液中前列腺素E2含量的影响

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11、

the effect of microinjection of penicillin g-k into arachnoid space at obex on the concentration of 5-ht in brain stem

脑闩蛛网膜下腔微量注射青霉素G-K对脑干组织5&羟色胺含量的影响

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12、

in 56 urethane anesthetized wistar rats, bilateral microinjection of glutamate ( lglu) was used to observe the difference of cardiovascular effects between caudal ventrolateral medulla pressor area ( cvmp) and rostral ventrolateral medulla pressor area ( rvmp).

雄性Wistar大鼠56只,乌拉坦麻醉,双侧脑区微量注射谷氨酸钠,观察延髓腹面尾端加压区(cVMP)与头端加压区(rVMP)心血管效应的差异。

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13、

an improved method of purification of dna fragment for microinjection

一种改进的显微注射用DNA片段的纯化方法

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14、

methods polysomnography ( psg) and nucleus amygdala microinjection.

方法多导睡眠描记和杏仁核微量注射。

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15、

Furthermore, combining information of our research , it is emphatically introduced about microinjection into chicken , s blastoderm.

其中, 结合本实验室的情况, 重点介绍了胚盘直接注射法.

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16、

A very small pipette used in microinjection.

一种小型的或年幼的隼.

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17、

The pain threshold was not influenced by microinjection of lidocaine into NRM or RN.

单独用利多卡因阻断双侧红核或中缝大核对痛阈无影响.

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18、

In addition , the evoked responses could be inhibited by intralateroventricular microinjection of atropine.

侧脑室注射阿托品抑制扣带回前部神经元内脏痛觉诱发反应.

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19、

Whole mount in situ hybridization and transgene via microinjection are two important methods in zebrafish study.

整胚原位杂交和显微注射转基因技术是斑马鱼基因功能研究中两种重要的研究方法.

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20、

Mathematical model of microinjection amount is founded through depth theory research to the microinjector.

本文通过对显微注射仪的深入研究,建立了注射量的数学模型.

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