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METHODS: We used the methods of G-banded karyotype analysis, DNA basic sequence analysis and Western blot and the others, and studied the chromosome karyotype and the status of p53 gene of teratocarcinoma PA-1 cell line which had been cultured for 407-445 passages for 20 years.

方法:采用G带核型分析、DNA碱基序列分析及western blot(蛋白印迹分析)等方法对经20余年407-445继代培养的PA-1细胞株染色体核型及p53基因状态进行了研究。

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METHODS The purified product was characterized through Western blot, terminal amino acid sequence analysis, mass spectrometry and in vivo bioactivity assay. MOLECULAR CLONING OF HUMAN STEM CELL FACTOR GENE We have been studying the expression of rhSCF in E.

方法通过蛋白质杂交,氨基酸末端序列分析,质谱分析及动物体内生物活性的研究,对人干细胞因子基因大肠杆菌表达产物&重组人干细胞因子(rhSCF)分子进行了鉴定。

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The result of western blot displayed that the molecular weight of hGM-CSF expressed by transgenic silkworm silk gland was 22 kD.

western blot结果显示,转基因家蚕丝腺表达的hGM-CSF的分子量为22kD。

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Methods Wistar rats were fed on selenium-deficient diets for 14 weeks. The changes of thioredoxin reductase activity, protein expression and gene expression were detected by biochemical methods, Western blot and Northern blot.

方法用低硒饲料喂养断乳2周的Wistar大鼠14周,分别采用生物化学方法、western blot和Northern Blot杂交检测心肌组织中TR的活性改变及TR蛋白、基因表达变化。

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Western blot was used to detect ANA profile.

免疫印迹法检测ANA谱。

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The titer and specificity of mAb was detected by ELISA and Western blot respectively. Tissue localization of antigen was detected by immunohistochemical staining.

用间接ELISA法测定mAb的效价,以western blot鉴定mAb的特异性、免疫组化染色法对mAb相应的抗原进行组织定位。

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The expression of tissue transglutaminase protein was detected by immunohistochemistry and quantitated by Western blot analysis. In situ transglutaminase activity was measured by immunofluorescence substrate incorporation.

用免疫组织化学、底物掺入免疫荧光法、Western印迹法分别观察肾内tTG蛋白分布、原位活性和蛋白水平变化;

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Tissue specificity assay of expression of human FSCB: The expression of FSCB in multiple tissue including cardiac muscle, lung, pancreas, spleen, muscle and testis was observed and its specific expression in testis was confirmed using the PAb by Western blot. 5.

人FSCB蛋白表达的组织特异性分析:以所获得的多克隆抗体通过western blot检测人体正常心、肺、胰、脾、肌肉和睾丸组织蛋白表达情况,确定该蛋白仅在睾丸组织内有表达。

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The level of the total cholesterol was detected by high performance liquid chromatography, and the expression of Caveolin-1 was detected by Western Blot.

高效液相色谱分析法检测细胞内总胆固醇(TC)含量;Western印迹法检测Caveolin-1蛋白的表达。

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Tested the expression of WISP-1 in protein level in G0 and Gx using Western blot and compared the disparities.

采用western blot从蛋白水平检测G0和Gx中WISP-1的表达水平及差异。

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Identification of cloned human OB gene product by both Western blot and SDS-PAGE electrophoresis

克隆人肥胖基因表达产物瘦素的分子量及特异性鉴定

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Expressions of type ⅰ and type ⅱ collagen detected by Western blot analysis during tibial fracture healing in denervate rats

大鼠胫骨骨折愈合过程中Ⅰ、Ⅱ型胶原蛋白的表达:失神经状态下western blot方法测定

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The expression of NGF and IL-1 β were observed respectively by Western blot in the lung, C_7-T_5 spinal ganglia and the corresponding spinal dorsal horn of all the groups.

用western blot方法分别检测各组豚鼠肺、C7~T5段脊神经节和相应节段脊髓背角NGF和IL-1β的表达。

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Methods: The alterations of NGF expression were observed by means of immunohistochemistry and Western blot in the lung, C_ 7-T_ 5 spinal ganglia and the corresponding dorsal horn of the spinal cord of all guinea pigs.

方法:利用免疫组织化学和Western印迹方法,观察生理盐水组、单纯致敏组、哮喘组和地塞米松组豚鼠肺及内脏感觉传入系统(C7T5脊神经节及对应的脊髓后角)NGF表达的变化。

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Analysis of Human Leukocyte Interferon by Semi-dry Gel Transfered Western Blot

应用半干胶转移免疫印迹法分析人白细胞干扰素

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Western blotting also demonstrated the immune specificity of the purified fusion protein.

WESTERN BLOTTING印迹表明纯化的融合蛋白具有特异的免疫反应特性。

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19、

The expressed products were analyzed by SDS PAGE, Western blots and fibrin dissolving activity tests.

表达产物进行SDS page、western blots以及溶纤维蛋白活性检测。

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20、

Use Western Blot to test objective gene expression.

采用western blot法检测目的基因质粒表达。

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