Cotyledons of Cucumis melo var.I were transformed with Agrobacterium tumefaciens ( LBA4404) carrying ACC deaminase gene and Kanamycin resistant gene ( Npt ⅱ, as report gene). Some regenerated Kanamycin resistant seedlings were obtained.

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In this paper, the antisense waxy gene has been transfered into indica maintainer lines through Agrobacterium-medialed transformation in order to reduce amylose content of the receptor varieties properly and then improve their rice quality.

本研究用根癌农杆菌转化技术将反义waxy基因导入籼稻保持系，以适当降低受体品种的直链淀粉含量，从而改良稻米品质。

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In order to optimize the transformation system and improve transformation efficiency, we analyzed certain influencing factors of maize by Agrobacterium-mediated transformation by gus gene transient expression.

本研究利用β-葡萄糖苷酶（GUS）基因的瞬时表达对农杆菌转化玉米的条件进行了探索性实验，期望优化农杆菌介导的玉米遗传转化体系，提高外源基因的转化效率。

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Agrobacterium tumefaciens-mediated Transformation of Rice with Insecticidal Bacillus thuringiensis Gene and Bioassay of the Transgenic Plants

农杆菌转化获得转B.t.基因水稻及其生物学鉴定

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Objective To construct plant expressing murine rotavirus antigen gene and transform it into Agrobacterium tumefaciens.

目的构建表达鼠源轮状病毒抗原基因vp4的植物载体及农杆菌转化。

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Optimization of Agrobacterium-mediated Transformation Using the Immature Embryos and Introduction of Genes Coding HMW-GS Derived from Alien Species in Common Wheat

小麦幼胚农杆菌转化体系优化和HMW-GS基因转化研究

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Construction of plant expression plasmid of fatty-acid alcohol oxidase gene from candida cloacae and its transformation into agrobacterium tumefaciens

Candida Cloacae源脂肪酸醇氧化酶基因的植物表达质粒构建及农杆菌转化研究

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Scientists have used Agrobacterium-mediated genetic transformation to generate transgenic plants, and this method depends on the Ti plasmid.

在过去的几十年间，研究人员用农杆菌转化法获得了多种转基因植物，该转化法依赖于农杆菌中存在的Ti质粒。

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The Study of Genetically Modified Oilseed Rape by Agrobacterium-About Bt and Cecropin

农杆菌转化Bt和抗菌肽融合基因获得转基因油菜的研究

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Many useful transgenic plants identified by PCR and Dot-blotting were obtained by using this protocol.

应用此农杆菌转化系统，获得了一批经PCR和点杂交鉴定的转基因植株。

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Wheat Agrobacterium-mediated Transformation with Different Variety Explants

小麦不同品种外植体的农杆菌转化方法的研究

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An Agrobacterium-Mediated Transformation System Applicable for Four Genotypes of Maize

适用于4种玉米基因型的农杆菌转化方法的探讨

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Study on the improving of wheat quality by pollen tube pathway transgene

花粉管通道法转基因改良小麦品质的初步研究

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Probing into Cytological Embryology Mechanism of Pollen-tube Pathway Transgenic Technology

花粉管通道法转基因技术的细胞胚胎学机理探讨

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Studies on the Influence Factors of Wheat Transformation in Pollen-tube Pathway

花粉管通道法转化小麦影响因素的研究

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Application of Pollen Tube Pass Method to Transgenic Technology of Cotton

花粉管通道法在棉花转基因上的应用

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Pollen-tube Pathway Method and Its Application in Tobacco Breeding

花粉管通道法及其在烟草育种中的应用综述

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Development of Transgenic Wheat with Galanthus navies Agglutinin Gene ( s gna) via the Pollen_tube Pathway

利用花粉管通道法获得转雪花莲凝集素基因（sgna）小麦

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The pollen-tube pathway transformation technique can bypass the usual tissue culture and simplify soybean transformation procedures, but it is restricted in transgenic soybean because no sufficient evidence such as Southern-blot and heredity data to support exogenic gene to integrate into chromosome of soybean.

花粉管通道法转化大豆避免了组织培养过程，操作简便经济，但由于缺少Southern blot及后代遗传学分析证明外源基因整合进植物染色体上，在大豆转基因育种方面受到了限制。

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Transgenic rice plant was obtained by transferring the herbicide resistant gene bar into rice line "E32" by pollen-tube pathway method.

利用花粉管通道法将抗Basta除草剂的bar基因导入水稻品系E32，获得转基因植株。

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After 15 years studied, it is indicating that the gene from sorghum not only integrated into wheat but also expressed by introduced exotic DNA from sorghum ( 6A/ 115) into normal wheat through the pollen tube pathway.

经过我们15年的悉心研究，通过花粉管通道法将高粱DNA导入小麦，结果来自高粱的外源基因不但整合到小麦染色体，而且得到了表达。

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